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ARS Home » Pacific West Area » Wapato, Washington » Temperate Tree Fruit and Vegetable Research » Research » Research Project #431023

Research Project: Molecular Gut Content Analysis to Pinpoint Where Psylla Overwinter

Location: Temperate Tree Fruit and Vegetable Research

Project Number: 2092-22430-002-24-T
Project Type: Trust Fund Cooperative Agreement

Start Date: Jul 2, 2016
End Date: Jul 1, 2018

Objective:
Design PCR primers to detect shelter plant DNA from pear psylla; Determine the number of sequences required to identify previous shelter hosts; Determine the half-life of plant DNA in pear psylla; Develop flight interception traps that can be used to trap returning psylla without compromising the plant (DNA) signal

Approach:
Design and test PCR primers to amplify DNA of a wide-variety of possible host plants visited by pear psylla. TA clone PCR products and sequence to determine shelter plant identity. Laboratory studies to determine how long the plant (DNA) signal persists in living pear psylla. Field studies to develop flight interception traps