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ARS Home » Plains Area » College Station, Texas » Southern Plains Agricultural Research Center » Crop Germplasm Research » Research » Research Project #430952

Research Project: Screening Xylella fastidiosa in the USDA ARS National Collection of Genetic Resources for Carya

Location: Crop Germplasm Research

Project Number: 3091-21000-042-02-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Jun 1, 2016
End Date: May 31, 2019

Objective:
1) Refine diagnostic procedures to insure identification of infection; 2) Refine sample collection protocols to optimize both phenotypic recognition and diagnostic identification; and 3) Screen collections of the NCGR-Carya to identify infected and disease free trees.

Approach:
1) Isolates of Xylella fastidiosa from previous work in pecan at LSU, Shreveport and TAMU, Uvalde will be sequenced; 2) New PCR primer sequences will be designed from the pecan Xf sequence and tested against the pecan genome to ensure that they do not bind non-specifically; 3) Detailed PCR conditions will be defined, specifying polymerase to be used; and 4) LAMP technique to identify Xf in other plant species will be evaluated using pecan DNA and pecan sap.