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United States Department of Agriculture

Agricultural Research Service

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Research Project: Immunity to Potato Cyst Nematodes

Location: Horticultural Crops Research

Project Number: 2072-22000-043-15-I
Project Type: Interagency Reimbursable Agreement

Start Date: Jul 1, 2016
End Date: Jun 30, 2017

Objective:
Experiments will be conducted to evaluate potato varieties/clones for resistance to G. ellingtonae

Approach:
Utilizes resistant potato lines, such as NY121, V-15-71, Karaka and Moonlight in hybridizations to incorporate resistance into russet and processing potato lines. Resistant lines will be identified and carried forward in the breeding program to generate advance breeding lines and cultivars. Increase seed of resistant lines for evaluation at ARS-Corvallis (G. ellingtonae). Sprouted tubers will be transplanted into 4” clay pots and roots inoculated with 2,500 eggs. Russet Burbank will be included as the susceptible control. Plants will be grown in a temperature-controlled greenhouse and fertilized. Plants will be allowed to grow for at least three months and will then be destructively harvested. At harvest, tops will be removed and discarded. The roots and soil will be place in a pan and air dried for 7 days after which roots will be cut into small pieces and roots and soil stored at 4 C until processing. Cysts will be extracted from the samples using a Fenwick can. Cysts will be picked from samples and enumerated. Collected cysts will be crushed to free eggs and the number of eggs determined. Reproduction factors (final population/initial population) will be calculated. The collaborators on this project will generate potato lines for screening (Novy and Whitworth) or will screen potato lines against G. pallida (Dandurand) or G. rostochiensis (Wang). Data will be shared among members of the group to enable decision making based upon data from 3 Globodera species.

Last Modified: 09/21/2017
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