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ARS Home » Plains Area » Lubbock, Texas » Cropping Systems Research Laboratory » Plant Stress and Germplasm Development Research » Research » Research Project #430900

Research Project: Molecular Characterization of Nematode and Fusarium Wilt Resistance in the Cotton Genome

Location: Plant Stress and Germplasm Development Research

Project Number: 3096-21000-022-03-N
Project Type: Non-Funded Cooperative Agreement

Start Date: Apr 1, 2016
End Date: Mar 30, 2019

Objective:
To evaluate diverse cotton germplasm with different levels of tolerance / resistance to root-knot nematode and fusarium wilt for identifying associations and fine mapping of genome regions.

Approach:
Acala, non-Acala Upland, Pima, and other Gossypium germplasm and progeny from a broad genetic background will be evaluated for root-knot nematode (RKN) and fusarium wilt (FOV) tolerance/resistance at the University University Riverside, CA. A minimum of two replications will be used for FOV resistance estimation when seed availability is limited in completely randomized or randomized complete block design studies. Measured responses specific to RKN and FOV studies will include stand survival rates, ratings of disease severity, foliar symptoms, and vascular staining. In addition, sequenced BAC clones will be analyzed for possible targets for resistance (R) genes and new marker development. R genes/markers will be evaluated and validated for marker–R gene associations by screening the markers on additional mapping populations from cotton crosses for which the progenies have been pathogen-resistance phenotyped. In addition, existing and new molecular-marker data will be generated (e.g., using the Illumina Cotton63K array) from evaluated germplasm and progeny segregating for pathogen resistant phenotypes, and data will be analyzed by statistical and genetic packages. Both qualitative and quantitative (QTL)-based mapping will be conducted to reveal markers associated with nematode and wilt resistance genes and their genetic positions in the R-gene cluster region.