Location: Healthy Body Weight Research
Project Number: 3062-51000-052-03-N
Project Type: Non-Funded Cooperative Agreement
Start Date: Sep 1, 2017
End Date: Dec 31, 2020
The purpose of this collaboration is to maintain cooperation and coordination of studies involving energy metabolism regulation by nutrients. Collaborating faculty from UND has colonies of long-living and short-living mice that are used to study interventions that impact health and longevity. Outcome measures include behavior (anxiety, spatial learning, memory, motor coordination, balance, grip strength), oxidative metabolism, amino acid metabolism, mitochondrial function and epigenetic marks. USDA-ARS-GFHNRC personnel are studying maternal diet-induced epigenetic alterations in obesity. ARS has studied offspring white and brown adipose, offspring skeletal muscle and liver, offspring skeletal muscle mitochondrial oxidative enzyme gene expression, and dam placental tissues. Studies have shown that mitochondrial dysfunction contributes to aging and that aging is associated with decreased skeletal muscle mass and increased adiposity. Whether maternal diets affect mitochondrial function to contribute to offspring aging, reduction in skeletal muscle mass and increased adiposity have not yet been determined. Therefore, the objective is to examine the impact of maternal nutritional interventions that alter energy metabolism in skeletal and adipose tissues during offspring development and aging processes.
ARS and UND personnel will use instruments at the Grand Forks Human Nutrition Research Center such as Pyromark 24 to sequence liver, adipose, and skeletal muscle genes involved in regulation of age-associated metabolic pathways that may be affected by epigenetic alterations. Other instruments such as Seahorse analyzer will be used to determine oxidative energy metabolism from the same tissues.