Location: Vegetable Crops Research
Project Number: 5090-21220-005-13-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Jul 1, 2016
End Date: Dec 31, 2018
Our specific objectives are: 1. Identify genetic polymorphisms within specific genes in Permier Russett (PR) and Russett Burbank (RB) using a novel high throughput RenSeq approach and use these markers to identify the relevant gene(s) that segregate(s) with resistance in a PRxRB population. 2. Use next-generation sequencing to quantify the expression of these genes in PR and RB and the expression level of a mutated transgene over that of endogenous alleles in our transgenic lines. 3. Correlation network analysis. This analysis will allow identifying networks in which these genes are involved, and will help in the identification of the resistance.
Cooperator will assist with data analysis, provide plant materials necessary to generate the segregating population, and provide data related to the correlation of gene expression data with known resistance responses. ARS will provide resources and methods for the analysis of resistance gene sequences within the population.