Location: Cereal Crops Research
Project Number: 3060-22000-050-039-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Aug 1, 2016
End Date: Apr 30, 2021
Objective:
The primary objective of this cooperative research project is to use a segregating mapping population of Pyrenophora teres f. teres to identify and characterize virulence factors contributing to net form net blotch disease on barley.
Approach:
A mapping population of P. teres f. teres will be used to identify virulence genes contributing to net form net blotch disease on barley. Phenotyping has been completed for a P. teres f. teres mapping population on ten barley lines that have been used as NFNB differential lines in several studies. This data has been used in conjunction with mapping data to identify both major and minor QTL associated with virulence. RNAseq data will be used to annotate the genomes of the parental isolates in this mapping population. Annotated genes within the QTL regions will be prioritized based on criteria typical of effector genes and evaluated using heterologous expression, gene transformation, and site directed gene disruption. Validated genes will be used in downstream application to look at this host pathogen interaction.
