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ARS Home » Northeast Area » Ithaca, New York » Robert W. Holley Center for Agriculture & Health » Plant, Soil and Nutrition Research » Research » Research Project #430667

Research Project: Dissecting the Molecular Mechanisms Underlying Chromoplast Biogenesis for Carotenoid Accumulation

Location: Plant, Soil and Nutrition Research

Project Number: 8062-21000-047-03-A
Project Type: Cooperative Agreement

Start Date: Apr 1, 2016
End Date: Oct 30, 2019

Objective:
Carotenoids are indispensable to plants and play a critical role in human nutrition and health. The massive carotenoid accumulation in many fruits and vegetables depends on chromoplast biogenesis. However, the intriguing molecular mechanisms underlying chromoplast biogenesis remain an enigma. The objective for the cooperative research is to test whether chromoplast biogenesis is mediated by OR via regulating proteins in the plastid protein import pathway.

Approach:
Standard genetic, molecular and biochemical approaches will be employed. They include the examination of plastid import protein stability by western blot analysis, the selective import of chromoplast preproteins by import assays, the selective binding of chromoplast preproteins by selective binding assays, and the plastid membrane proliferation by microscope and biochemical analyses.