1. Novel selective, bacteriological medium formulated to support growth of Campylobacter. ARS researchers in Athens, Georgia, formulated a new, selective bacterial medium that allows scientists and technicians to grow Campylobacter in containers incubated aerobically. Campylobacter is a major, bacterial foodborne pathogen associated with the consumption of contaminated poultry products and other foods. However, research on the bacterium is limited because the pathogen must be grown under artificial atmospheres on media supplemented with blood. The new medium does not require the utilization of artificial atmospheres or blood. Furthermore, the addition of selective agents to the medium enables researchers to isolate the bacterium from environmental samples that contain other microorganisms. The novel medium will simplify procedures and reduce costs required to grow the pathogen; thereby, increasing the number of laboratories able to conduct research on this bacterium.
2. Isolation and identification of amylase-producing, endospore-forming bacteria for use in defined probiotic cultures. ARS researchers in Athens, Georgia, isolated amylase-producing, endospore-forming bacteria from the intestinal tract of commercially processed broiler carcasses. Probiotics are cultures of beneficial bacteria that are provided to live poultry to reduce the colonization of the birds by foodborne pathogens, such as Salmonella. Including bacteria that produce the enzyme, amylase, in probiotic cultures might improve the efficacy of these products because amylase can breakdown starch in poultry feed to produce simple sugars that can be used by other beneficial bacteria. The amylase-producing bacteria were identified because regulatory agencies only approve the use of probiotics of known bacterial composition. More effective probiotics will reduce the number of human pathogens carried by live poultry; thereby, reducing the number of illnesses associated with the consumption of contaminated poultry.
3. Development of neutralizing Buffered Peptone Water (nBPW) to inactivate chemical sanitizers in Salmonella verification studies. ARS researchers in Athens, Georgia, collaborated with the Food Safety Inspection Service (FSIS) to develop a neutralization solution (nBPW) for use by commercial poultry processors in Salmonella verification testing. Commercial poultry processors use chemical sanitizers during processing to reduce contamination of carcasses by human foodborne pathogens. However, if traces of these sanitizers are carried-over into testing samples used to determine contamination of poultry carcasses and parts by Salmonella, the results of these tests may be inaccurate. The utilization of the nBPW improves the accuracy of Salmonella verification testing by inactivating trace-amounts of the sanitizers in the test samples. On July 1, 2016, the FSIS informed inspectors in commercial poultry processing facilities that nBPW should be used in all verification testing, and the FSIS is currently seeking a commercial vendor to produce nBPW. The utilization of nBPW will improve the accuracy of verification testing and will provide regulatory agencies and food safety researchers with reliable data to develop methods to reduce contamination of processed poultry by foodborne pathogens.
4. Broiler cecal and respiratory tracks Salmonella. ARS researchers in Athens, Georgia, sampled commercial broilers the week prior or the day prior to catching for transport to the processing plant and revealed that initial cecal sampling results fail to accurately predict Salmonella prevalence following feed withdrawal. Respiratory tracts are not significantly contaminated with Salmonella, aerobic, or Enterobacter bacteria following house preparation and catching. These results further complicate the practical implication of logistic slaughter by attempting to process negative and false negative flocks first each processing day.
5. Electrically stunning shackled broilers. ARS researchers in Athens, Georgia, in 2016 demonstrated that electrically stunning shackled broilers using a combination of pulsed DC (salt-water bath for 10 seconds) followed by the application of AC (contact plate for 5 seconds) confirmed 100% efficacy in induction of a non-recoverable stun-to-death. In 2017 ARS researchers in Athens, Georgia, sampled and confirmed that there were no significant differences for broiler whole carcasses (hemorrhages, red wing tips, broken clavicles) and breast or leg meat quality characteristics (meat pH, cook loss, redness and yellowness color values, and instrumental tenderness) between control and the stun-to-death treatment groups (DC+AC combination stunning). These data indicate that these stunning parameters combining DC and AC stunning should be viable protocols when a stun-to-death is desired. Commercial processing plants can utilize this combined DC+AC stun for any customers that may require assurance of an irreversible electrical stun at slaughter.Bourassa, D.V., Wilson, K.M., Bartenfeld, L.N., Harris, C.E., Howard, A.K., Ingram, K.D., Hinton Jr, A., Adams, E.S., Berrang, M.E., Feldner, P.W., Gamble, G.R., Frye, J.G., Jackson, C.R., Johnston, J.J., Buhr, R.J. 2017. Surface water accumulation and subsquent drip loss for processed broiler carcasses subjected to a post-chill water dip or spray. Poultry Science. 96(1):241-245.
Crespo, M.D., Kathariou, S., Grimes, J.L., Cox Jr., N.A., Buhr, R.J., Frye, J.G., Miller, W.G., Jackson, C.R., Smith, D.P. 2016. Routes of transmission of Salmonella and Campylobacter in breeder turkeys. Journal of Applied Poultry Research. 25(4):591-609.
Bourassa, D.V., Kannenberg, E.L., Sherrier, D.J., Buhr, R.J., Carlson, R. 2017. The lipopolysaccharide lipid-a long chain fatty acid is important for rhizobium leguminosarum growth and stress adaptation in free-living and nodule environments. Molecular Plant-Microbe Interactions. 30(2):161-175. doi.org/10.1094MPMI-11-16-0230-R.
Gamble, G.R., Berrang, M.E., Buhr, R.J., Hinton Jr, A., Bourassa, D.V., Johnston, J.J., Ingram, K.D., Adams, E.S., Feldner, P.W. 2017. Neutralization of bactericidal activity related to antimicrobial carry-over in broiler carcass rinse samples. Journal of Food Protection. 80(4):685-691. doi:10.4315/0362-028x. JFP-16-412.
Cox Jr, N.A., Richardson, K., Cosby, D.E., Berrang, M.E., Cason, J., Rigsby, L.L., Holcom, N., Derome, L. 2016. Injury and death of various Salmonella serotypes due to acidic conditions. Journal of Applied Poultry Research. 25(1):62-66.
Landrum, M.A., Cox Jr, N.A., Cosby, D.E., Berrang, M.E., Russell, S.M. 2016. Treatment with a low pH processing aid to reduce campylobacter counts on broiler parts. Poultry Science. 96:1028-1031.
Adhikari, P., Cosby, D.E., Cox Jr, N.A., Kim, W.K. 2017. Colonization of mature laying hens with salmonella enteritidis by oral or intracloacal inoculation. Journal of Applied Poultry Research. 26(2):286-294. doi.10.3382/japr/pfw072.
Berrang, M.E., Cox Jr, N.A., Cosby, D.E., Frye, J.G., Jackson, C.R. 2016. Detection of Salmonella serotypes by overnight incubation of entire broiler carcass. Journal of Food Safety. doi: 10.1111/jfs.12298.
Berrang, M.E., Meinersmann, R.J., Ladely, S.R., Cox Jr, N.A. 2017. Campylobacter detection in broiler ceca at processing - a three year 211 flock survey. Journal of Applied Poultry Research. 26:154-158.
Cox Jr, N.A., Richardson, L., Cosby, D.E., Berrang, M.E., Wilson, J.L., Harrison, M.A. 2016. A four-quadrant sequential streak technique to evaluate Campylobacter selective broths for suppressing background flora in broiler carcass rinses. Journal of Food Safety. doi:10.1111/jsf.12311.
Ladely, S., Berrang, M.E., Meinersmann, R.J., Cox Jr, N.A. 2017. Campylobacter multi-locus sequence types and antimicrobial susceptibility of broiler cecal isolates: a two year study of 143 commercial flocks. Journal of Food Safety. doi:10.1111/jfs.12366.
Zhuang, H., Bowker, B.C., Berrang, M.E., Meinersmann, R.J., Buhr, R.J. 2017. Impact of eliminating the carcass chilling step in the production of pre-cooked chicken breast meat. Journal of Applied Poultry Research. 26(3):431-436. doi.org/10.3382/japr/pfx012.
Bourassa, D.V., Bowker, B.C., Zhuang, H., Wilson, K.M., Harris, C.E., Buhr, R.J. 2017. Impact of alternative electrical stunning parameters on the ability of broilers to recover consciousness and meat quality. Poultry Science. 96(9):3495-3501. doi:10.3382/ps/pex120.
Berrang, M.E., Ladely, S.R., Meinersmann, R.J., Line, J.E., Oakley, B., Cox Jr, N.A. 2016. Variation in Campylobacter multilocus sequence subtypes from chickens as detected on three plating media. Journal of Food Protection. 79(11):1986-1989.