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ARS Home » Northeast Area » Orono, Maine » New England Plant, Soil and Water Research Laboratory » Research » Research Project #430604

Research Project: Advancing the Technologies in Detecting, Diagnosing, and Controlling Black Leg Disease (Dickeya spp.) in the U.S.

Location: New England Plant, Soil and Water Research Laboratory

Project Number: 8030-12210-001-02-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Apr 15, 2016
End Date: Oct 1, 2018

Objective:
Determine the genetic background and distribution of Dickeya and Pectobacterium isolates.

Approach:
A survey of potato seed producers in both the lower and upper peninsula of Michigan, as well as from black leg infected plants in commercial production fields during the growing season. Michigan State University will work with the Michigan Potato Industry Commission, the Michigan Seed Potato Association and Michigan Department of Agriculture and Rural Development to coordinate this effort. Incidence of infected tubers will be counted. Dickeya and Pectobacterium isolates, will be obtained from multiple locations in Michigan. Isolations will be made on crystal violet pectin (CVP) medium and a general nutrient medium. Pathogenicity will be assessed with a potato tuber bioassay. DNA of the isolates will be extracted, and will be used in multiple analyses in the characterization of the bacterial isolates. Genes of interests will be amplified using PCR and the resulting products will be sequenced for pathogen identification and classification. PCR amplicons will be generated using the ADE1/ADE2 primers of Nassar et al. (1996) and the genus level primers of Potrykus et al. (2014). Sequencing of the products will be pursued to clarify the relationship among isolates. Alternative (large scale) sequencing strategies to generate MLST relationships among isolates will be evaluated.