Location: Agroecosystems Management Research
Project Number: 5030-13000-011-51-R
Project Type: Reimbursable Cooperative Agreement
Start Date: Feb 1, 2016
End Date: Aug 1, 2019
1. Determine the effect of application timing on losses of antibiotics and antibiotic-resistance genes from manured fields in tile drainage water. 2. Determine the effects of alternative manure treatment and storage on the persistence of antibiotic-resistant bacteria and antibiotic-resistance genes in manure.
Losses of antibiotics and antibiotic-resistant bacteria will be determined in runoff and tile drainage at existing field sites, which receive swine manure applied in the fall, in the spring in alternate years, or every year. The antibiotics, tylosin, tetracycline and sulfamethazine, are the focus of the study, and these will be measured in soil and water by extraction and HPLC-MS/MS. Antibiotic-resistance will be assessed by measuring antibiotic-resistance genes relevant to the focus antibiotics using qPCR methods. In addition, we will evaluate the effects of iopophore treatment of manure, manure storage without manure addition, and heat treatment on persistence of antibiotic-resistance genes.