Location: Grain Quality and Structure Research
Project Number: 3020-43440-001-08-I
Project Type: Interagency Reimbursable Agreement
Start Date: Feb 1, 2017
End Date: Jan 31, 2019
The overall objectives of this project are: Objective 1: Find new sources of high carotenoid sorghum by characterizing the natural variation in sorghum grain carotenoids in a large diverse panel . Objective 2: Identify genetic markers associated with natural variation of sorghum grain carotenoids. Objective 3: Validate putative functional alleles underlying genetic markers of carotenoid variation.
The main objectives will be achieved as follows. For objective 1, High-performance liquid chromatography (HPLC), spectrophotometry, and colorimetry will be used to quantify endosperm color and total carotenoids, and identify and quantify the concentration of seven carotenoid types: apocarotenal, zeaxanthin, beta-cryptoxanthin, beta-carotene, alpha-carotene, lutein, and lycopene. For objective 2, Genome-wide association studies (GWAS) and linkage mapping will be used to map loci controlling quantitative carotenoid variation, presence/absence of carotenoids, and endosperm color. For objective 3, PCR primers will be designed for conducting allele specific sequencing of candidate genes to identify causal polymorphisms.