Location: Plant, Soil and Nutrition Research
Project Number: 8062-21000-046-07-A
Project Type: Cooperative Agreement
Start Date: Jan 1, 2016
End Date: Jun 30, 2018
1) Characterizing natural variation in the Al tolerance gene ART1, and how it can be exploited to improve rice Al tolerance. 2) Take advantage of the data from the joint linkage-genome wide association analysis of rice Al tolerance to identify novel rice Al tolerance genes and their corresponding physiological mechanisms.
We will study the effect of the ART1 alleles from the tolerant and sensitive parents of our RIL population on Al tolerance and global gene expression in different genetic backgrounds. We also will search for interesting ART1 alleles in our diversity panel, functionally test them in a heterologous system, try to identify functional polymorphism(s) that affect ART1 function, and test candidate alleles via rice transformation. We will also investigate ART1 regulation by searching for ART1-interacting proteins. Characterize cell wall-related genes identified within a genomic interval associated with Al tolerance based our GWAS results. Characterization of candidate genes will be coordinated with the profiling of cell wall polysaccharide composition in a panel of Al-tolerant and sensitive rice lines.