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ARS Home » Plains Area » Manhattan, Kansas » Center for Grain and Animal Health Research » Hard Winter Wheat Genetics Research » Research » Research Project #429778

Research Project: Tolerance to Environmental Stress in Wheat

Location: Hard Winter Wheat Genetics Research

Project Number: 3020-21000-011-10-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Sep 1, 2015
End Date: Aug 30, 2020

1) Develop population to map genes for heat tolerance in the cross MT1020/Granger. 2) Conduct association mapping for heat tolerance on the cross OK06114 x Wendy. 3) Collect phenotyping for heat tolerance on a spring wheat association mapping panel. 4) Conduct fine mapping of wheat preharvest sprouting tolerance gene in cultivar Totumai develop diagnostic markers for marker-assisted selection.

Recombinant inbred populations, doubled haploid populations, or near isogenic lines will be developed to map and evaluate environmental stress tolerance genes or sprouting tolerance genes. High throughput genotyping will use either the 90K iSelect platform or genotyping-by-sequencing (GBS). The resulting lines or populations will be phenotyped for reactions to environmental stress in the growth chamber, field, or greenhouse. The chromosomal locations of stress tolerance and sprouting tolerance genes will be determined by association mapping or quantitative trait locus (QTL) analysis.