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ARS Home » Plains Area » Manhattan, Kansas » Center for Grain and Animal Health Research » ABADRU » Research » Research Project #429599

Research Project: Virus and Vector Protein Expression

Location: Arthropod-borne Animal Diseases Research

Project Number: 3020-32000-010-05-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Sep 1, 2015
End Date: Aug 31, 2018

Objective:
The objective of this collaborative project is the batch expression of multiple proteins of virus or insect vector origin. These proteins will be biologically characterized and used in diverse downstream assays. The ultimate goal is to support development of diagnostic assay, experimental serology and countermeasure development. The assays developed will be very useful in investigating mechanisms of virus transmission and disease pathogenesis/virulence. Emerging vector-borne diseases are especially important because of their high frequency of emergence, their economic impact on the global livestock industries, and their zoonotic potential.

Approach:
ABADRU will provide sequences (and/or a construct in some cases) of the following proteins for preliminary expression and subsequent batch production in appropriate constructs: Culicoides (Baculovirus expression) D7 (~500 bp) Late trypsin (~800 bp) Maltase (~1800 bp) BTV (E. coli expression) VP-2’s of domestic serotypes 2, 10, 11, 13, 17 (~3 Kb) for sero-diagnostics and antibody production VP-7 of BTV-11 (~1200 bp) for antibody production, cross-reactive for all BTV serotypes EHDV (E. coli expression) VP-2’s of domestic serotypes 1, 2, 6 (~3 Kb) for sero-diagnostics and antibody production VP-7 of EHDV-2 (~1200 bp) for antibody production, cross-reactive for all EHDV serotypes Other protein projects may be added as resources and finances allow.