Location: Sunflower and Plant Biology Research
Project Number: 3060-21220-031-010-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Jul 1, 2015
End Date: Dec 31, 2019
Objective:
Conduct research on Objectives 1 and 3 per the approved NSI project plan for the Oregon State University components:
1. Identify, transfer and validate white mold (WM) resistance factors from P. coccineus and P. vulgaris in advanced snap bean breeding lines.
3. Conduct genome-wide association (GWAS) mapping in Snap Bean, Middle American and Andean Diversity Panels using genotype-by-sequencing (GBS).
Approach:
Objective 1: Lines developed from inbred backcross populations from P. vulgaris //P. vulgaris x P. coccineus crosses will be used to identify QTL associated with WM resistance. Those QTL will be validated in separate populations and environments to determine if they are broadly expressed in multiple populations. The P. coccineus QTL will be transferred into advanced common bean breeding lines (both snap and dry beans) with stable fertility traits and adequate agronomic performance. New sources of resistance in P. vulgaris identified in the Snap Bean Diversity Panel (SBDP) through GWAS will be crossed to susceptible lines to create recombinant inbred (RI) populations to characterize potentially novel QTL for resistance.
Objective 3: Genotype-by-sequencing (GBS) will increase the genome-wide association (GWAS) mapping resolution for Snap Bean, Mesoamerican (syn Middle American), and Andean Diversity Panels (SBDP, MDP, and ADP), respectively. The three association mapping populations represent accessions grouped by origin, (MDP representing pinto, Great Northern, medium red, pink, navy, and black market classes; ADP representing kidney, cranberry) and utilization (snap beans). Additional white mold disease reaction data from greenhouse and field trials will be collected on the populations across locations and years, and GWAS analysis will discover significant QTL by association with high resolution (<50 kb).
