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ARS Home » Plains Area » Manhattan, Kansas » Center for Grain and Animal Health Research » ABADRU » Research » Research Project #429120

Research Project: Development and Evaluation of Diagnostic Applications for Arboviruses

Location: Arthropod-borne Animal Diseases Research

Project Number: 3020-32000-010-07-N
Project Type: Non-Funded Cooperative Agreement

Start Date: Aug 1, 2015
End Date: Jun 30, 2018

Objective:
The long term goal of this project is the development of rapid, reliable, and sensitive diagnostic assays for the identification of insect transmitted viruses, arboviruses, that are suitable for use in veterinary diagnostic laboratories, as well as in the field for use during times of natural or intentionally caused disease epidemics. Ultimately these assays can be evaluated as part of multi-agent, syndromic diagnostic disease panels for veterinary diagnostic medicine.

Approach:
The ARS and KSU have and are developing assays for detecting genetic material, antigen or antibodies to viruses using a variety of diagnostic formats on a manual laboratory scale. The approach is to evaluate these assays on diagnostic specimens in conjunction with the standard assays in use in the Kansas State Veterinary Diagnostic Laboratory (KSVDL). Those assays that are found to be acceptable then will be further optimized, validated and automated (as appropriate for the specific assays) for use in the diagnostic laboratory and potentially for commercial development. The primary focus of the proposed studies will be the demonstration and evaluation of new diagnostic assays for arboviruses, followed by the translation of these to validated tests to be used by veterinary diagnosticians. This work may include collaborative research projects lead by ABADRU and/or KSVDL scientists. The research work may also include consultation, professional diagnostic services, specimen testing, laboratory research investigations and data analysis/reporting. Project-by-project agreements will be developed as needed to secure financial, material and intellectual property questions, specific to the technical nature of each project.