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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Cereal Crops Research » Research » Research Project #429045

Research Project: GWS to Develop ND Elite Malting Barley with Disease Resistance Package Including Ug99 Resistance

Location: Cereal Crops Research

Project Number: 3060-21000-038-04-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Jul 1, 2015
End Date: Jun 30, 2020

Objective:
The primary objective of this cooperative research project is to rapidly pyramid and deploy the RMRL and RpgSw645 Pgt race TTKSK resistance loci with Fusarium head blight, spot form net blotch, net form net blotch, and spot blotch resistances into agronomically advanced Midwestern 2-row malting barley cultivars.

Approach:
The RMRL locus has been introgressed into the Conlon background and shown to confer resistance to the virulent stem rust race TTKSK at the adult plant stage in the Kenya, Africa field nursery. These lines were top crossed with the Swiss landrace Hv645, which contains the RpgSw645 adult plant resistance. A Harrington x Hv645 F7 recombinant inbred line (RIL) population was developed and phenotyped in the Kenya Ug99 nurseries for two seasons. We have genotyped this population with a 384 PCR-GBS SNP panel that was developed with markers spread evenly throughout the genome. However, this SNP panel developed from the Illumina Infinium iSELECT 9k SNP data mined from the Triticeae coordinated agriculture project (T-CAP) Triticeae Toolbox (T3) website (www.triticeaetoolbox.org) for a Tradition x PI67381 population yielded only 84 polymorphic markers. Thus, we will utilize a second PCR-GBS panel containing an additional 418 SNPs on the Harrington x Hv645 RIL population to further saturate the genetic map with markers. We have analysis with the current low resolution markers showing a significant peak on chromosome 5H at position 33 cM to 46 cM flanked by the SNP markers 11_10621 and 12_30745. We expect that saturating the population with more SNP markers will delimit the region even further, providing markers for selection of RpgSw645 in the Conlon/HQ1 BC4F3/SWISS 645/Conlon F2 individuals, which we will be screening in the field in 2015 and making Conlon backcrosses for further screening in the greenhouse in the fall of 2015. The Conlon/HQ1 BC5F3:4 lines with the highest levels of TTKSK resistance (FAR14-94A-1, FAR14-95A-2, FAR14-95A-3 and FAR14-95A-4) have been planted in yield trials in Fargo, ND to determine their yield as well as measure malt quality. The best lines will be provided to the NDSU barley breeder (Dr. Richard Horsley) and any other interested barley-breeding programs as prebreeding lines. We will continue improving upon these germplasms by complementing them with a wide fungal disease resistance package and introgressing the resistance loci into the new elite 2-row malting variety ND-Genesis recently released by the NDSU breeding program. The use of our PCR-GBS GWS panels now allows us to genotype progeny lines in a very high throughput manner for a cost of ~ $7 per line. However, we recently developed a protocol to recycle our PGM 318 microprocessor chips that reduces the cost to $4 per line.