1a. Objectives (from AD-416):
Objective 1: Investigate key biotic factors influencing the spatial and temporal dynamics of wood-boring pest (including ALB and EAB) populations in their native range, focusing on exploration and quarantine service for effective, host-specific natural enemies (parasitoids) for biocontrol. Subobjective 1a - Explore for parasitoids of ALB and EAB in the pests’ native range (Asia). Subobjective 1b - Evaluate the role of the key natural enemies in regulating the spatial and temporal dynamics of ALB and EAB in the area of collection in Asia. Subobjective 1c - Evaluate the host specificity of parasitoids discovered for introduction to North America for ALB and EAB control. Subobjective 1d - Quarantine services to support research on exotic insect pests and their natural enemies. Objective 2: Conduct field releases and evaluate impacts of extant (indigenous) and previously introduced parasitoids on populations of wood-boring beetles such as EAB and ALB in the United States, while elucidating factors that influence successful establishment of introduced biological control agents, such as climate adaptation, release methodology, genetic variation in founder populations and risk-spreading (diapause) strategy. Objective 3: For newly discovered parasitoids of ALB, EAB and other invasive pests, and based on studies of life histories and reproductive biology, develop effective rearing technologies for these natural enemies, focusing on optimizing host stage, host substrate complex, temperature, photoperiod and relative humidity. Subobjective 3a - Characterize the reproductive biology, risk-spreading (diapause) strategy and key life history parameters of most promising parasitoid species. Subobjective 3b - Determine the optimal host stage and host-substrate complex in association with host density and host-to-parasitoid ratio. Subobjective 3c - Determine the optimal environmental conditions for adult parasitoid survival, oviposition and progeny development including diapause induction and termination.
1b. Approach (from AD-416):
Using the approaches relevant to the knowledge base of the targeted pests and their natural enemies, we will conduct foreign explorations for new natural enemies from the pests’ native home (Northeast Asia) and construct life tables of the target pest populations to evaluate the impact of the natural enemies on the pests’ population dynamics in Northeast Asia. After selecting the most promising (or efficient) natural enemies, we will test the selected natural enemies against non-target wood-boring insects in North America to delineate their host range for biological control introduction against the target pests. Upon regulatory approval for environmental releases of the discovered natural enemies in North America, we will conduct field experiments to assess their establishment, dispersal and impacts on the target pests’ population in the U.S. Laboratory studies will also be conducted to collect information on the parasitoid’s biology, risk-spreading and reproductive strategy and life history and to develop efficient rearing methods for mass-production of the introduced natural enemies for biological control releases. In addition, this project will provide quarantine services, host range data and mass-rearing technologies for natural enemies of high priority plant pests to state and federal agencies.
3. Progress Report:
No progress made; see annual/final report for 8010-22000-023-00D.