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ARS Home » Northeast Area » Kearneysville, West Virginia » Appalachian Fruit Research Laboratory » Innovative Fruit Production, Improvement, and Protection » Research » Research Project #428683

Research Project: Translateome Alterations Affecting Virus Spread and Disease Development in Stone Fruit Crops

Location: Innovative Fruit Production, Improvement, and Protection

Project Number: 8080-21000-029-04-R
Project Type: Reimbursable Cooperative Agreement

Start Date: Feb 1, 2015
End Date: Jan 31, 2019

Objective:
The objectives for this project will be: 1) investigate translateome alterations that occur within the vascular tissues of plum (Prunus domestic L.) infected with Plum Pox virus (PPV), 2) identify phloem specific alterations that occur as a general response to virus infection, and 3) investigate the function of virus induced phloem genes in the movement and development of disease symptoms.

Approach:
A novel translateome profiling method that incorporates phloem specific promoters to express FLAG-tagged ribosomal proteins for immuno-capture of cell-specific mRNAs will be used to identify vascular specific transcripts that are altered in response to PPV as well as specific viral RNAs that are actively being translated. Analysis of the vascular tissues derived from root, shoot, bud and leaf at different seasonal time points (growth through dormancy) will produce a detailed understanding of PPV movement and its effects on phloem. Detail information derived from the PPV analysis outlined in Objective 1 will be used to guide additional phloem targeted translateome profiling studies using plum trees infected with either Prunus necrotic ringspot virus (PNRSV) or Tomato ringspot virus (ToRSV). Candidate genes that display differential regulation in response to PPV, PNRSV, and ToRSV infections will be further investigated within a N. benthamiana system by either gene knockdown using a virus-induced gene silencing (VIGS) system or over-expression using a 35S agro-infiltration system.