Location: Dairy and Functional Foods Research
Project Number: 8072-41000-102-06-N
Project Type: Non-Funded Cooperative Agreement
Start Date: Apr 1, 2015
End Date: Mar 31, 2018
The objective of this collaborative research is to evaluate the changes in composition, structure, and biological activities of ingested substances during the digestion process, as well as the mechanisms, by which ingested substances, such as foods, bacteria, chemicals and other external materials, influence the ecology and metabolism of the gut microbiota and the biology of the gut tissues along with their subsequent impact on health and nutrition.
Food and non-food substances will be fed to the TWIN-SHIME apparatus that mimics the gastrointestinal (GI) tract. Specimens will be collected at pre-determined time intervals and at various sites of the artificial in vitro GI tract for (1) analysis of the composition, structure, and biological activities of the ingested substances, (2) examination of the ecology and the change in diversity of the gut microbiota, (3) determination of the metabolites of the gut microbiota caused by the ingested substances, and (4) evaluation of the interactions between the metabolites and intestinal epithelium tissues. Chemical and biological analyses will be conducted using chromatography, mass spectrometry, microscopy, genomics assays, such as gene expression and sequencing (including RNAseq), and in vitro tissue cultures will be applied for the research.