Project Number: 2050-21000-034-06-T
Project Type: Trust Fund Cooperative Agreement
Start Date: Jul 1, 2015
End Date: Apr 30, 2019
This proposed project will: 1) Validate molecular genetic markers thought to be tightly linked to known crown rust resistance genes; 2) Create a minimally sized panel of markers sufficient to identify the allelic state of 4 to 10 resistance genes; and 3) Characterize oat lines submitted by breeders for allelic state of the crown rust resistance genes in the panel.
1. Marker Development. PCR-based assays will be developed for seedling- and adult plant-resistance genes with published locations and/or markers. Additional genes are expected to be identified with the genome-wide association mapping (GWAM) in progress. Bi-parental populations now under development will be used for linkage mapping of currently effective genes. All markers will be converted to a uniform technology platform. 2. Marker Validation. Markers will be evaluated for their ability to correctly classify oat lines for resistance genes by evaluating them against a set of Puccinia coronata isolates of known virulence. Each marker will have an experimentally-derived confidence rating. 3. Germplasm Characterization. Oat lines submitted by breeders will be characterized for number and identity of crown rust resistance alleles. This will be an ongoing process and is expected to increase in scope as the project progresses.