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ARS Home » Southeast Area » Athens, Georgia » U.S. National Poultry Research Center » Bacterial Epidemiology & Antimicrobial Resistance Research » Research » Research Project #428338

Research Project: Protozoa Capture Using a Microfluidic Device

Location: Bacterial Epidemiology & Antimicrobial Resistance Research

Project Number: 6040-41420-005-001-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Jul 1, 2015
End Date: Sep 30, 2017

Objective:
The objective for this project is to design, fabricate and test a microfluidic device to isolate and capture listeria-responsive protozoa. Use of protozoa that prey upon bacteria as a means to reduce the incidence of food-borne pathogens in food-processing plant has been recently explored at USDA-ARS. Georgia Tech Applied Research Cooporation and the Agricultural Research Service (ARS) desire to enter into this Agreement for the purpose of supporting research to be carried out at ARS and Cooperator facilities. ARS desires the Cooperator to provide research of mutual interest within the U.S. National Poultry Research Center.

Approach:
A microfluidic device, developed at Georgia Tech Research Institute (GTRI) will be used to study bacteria and protozoa interactions. The microfluidic device consists of a center channel that delivers the protozoa sample from a small reservoir to the central interaction region. The channels will be fabricated from the nitrocellulose membrane using a rapid prototyping technique. The device will be compatible with microscope platforms for flow and cell observations. The Location is engaged in research including Microbial Ecology of Human Pathogens Relative to Poultry Processing.