Project Number: 5012-21000-030-02-R
Project Type: Reimbursable Cooperative Agreement
Start Date: Jan 1, 2015
End Date: Dec 31, 2018
1) Produce soybeans lacking the redox-regulated a-subunits of activase and determine the impact on photosynthesis at the leaf and canopy levels; and 2) Characterize the phosphorylation of activase subunits in Arabidopsis and soybean.
Targeted genome editing using TALENs will be used to knockout the soybean a-subunit (redox-regulated) activase genes. The photosynthetic performance of transgenic plants will be assessed at the leaf level in response to changing light environment and if results are encouraging, in the field. We will also follow up our preliminary results that Arabidopsis activase is phosphorylated a Thr site in low light and in the dark, but not at high light. We will determine whether phosphorylation contributes to the inhibition of activase activity by direct measurement and by assessing the photosynthetic performance of plants expressing directed mutants at the position. With this base of knowledge, we will explore the phosphorylation of soybean activase by immunoblotting and LC-MS/MS analysis of SDS-PAGE purified protein. The results obtained would guide future soybean genome engineering strategies.