Location: Emerging Pests and Pathogens Research
Project Number: 8062-22000-022-001-R
Project Type: Reimbursable Cooperative Agreement
Start Date: Jun 15, 2015
End Date: Jun 14, 2020
Objective:
Develop U.S. potato germplasm with resistance to Globodera rostochiensis.
Approach:
Potato clones and cultivars will be planted in 3-inch pots that contain 1:2 v/v loam:sand mixture and grown in the greenhouse at 20-24 degrees C under a 16 h day length. When plants reach around 10 cm in height, they will be inoculated with 5,000 eggs of G. rostochiensis. Each line will have 3 replicates and both susceptible and resistant potato varieties (e.g., Pike and Katahdin) will be used as positive and negative controls. The potted plants will be harvested at 90 days after nematode inoculation and nematode cysts will be extracted from air-dried soil. Nematode reproduction will be analyzed to determine if the tested lines confer resistance to G. rostochiensis.
