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ARS Home » Northeast Area » Wyndmoor, Pennsylvania » Eastern Regional Research Center » Dairy and Functional Foods Research » Research » Research Project #427561

Research Project: Isolating and Characterizing Lactic Acid Bacteria from Buffalo Milk

Location: Dairy and Functional Foods Research

Project Number: 8072-41000-096-04-N
Project Type: Non-Funded Cooperative Agreement

Start Date: Sep 14, 2014
End Date: Sep 13, 2018

Objective:
1) Identify indigenous lactic acid bacteria (LAB) populations in raw buffalo milk. 2) Isolate LAB strains from buffalo milk and screen for potential bioactive compounds. 3) Isolate and identify bioactive compounds and assess their nutritional or biological effects in comparison to bovine milk.

Approach:
Raw buffalo milk samples will be screened using selective agar media for isolation of lactic acid bacteria (LAB). The LAB isolates will be screened for potential bioactivity. Strains exhibiting biological activity will be grown in pure cultures, and the resulting cell-free supernatants will be analyzed for the presence of active compounds. Bioactive molecules will be isolated and further characterized using protein analysis techniques to study their functional and/or nutritional properties.