Research Project: Technologies for Improving Process Efficiencies in Biomass Refineries

Location: Bioenergy Research

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Sugar production from bioenergy sorghum by using pilot scale continuous hydrothermal pretreatment combined with disk refining - (Peer Reviewed Journal)
Chen, M.H., Dien, B.S., Lee, D.K., Singh, V. 2019. Sugar production from bioenergy sorghum by using pilot scale continuous hydrothermal pretreatment combined with disk refining. Bioresource Technology. 289:121663. https://doi.org/10.1016/j.biortech.2019.121663.

Improving ethanol yields with deacetylated and two-stage pretreated corn stover and sugarcane bagasse by blending commercial xylose-fermenting and wild type Saccharomyces yeast - (Peer Reviewed Journal)
Wang, Z., Dien, B.S., Rausch, K.D., Tumbleson, M.E., Singh, V. 2019. Improving ethanol yields with deacetylated and two-stage pretreated corn stover and sugarcane bagasse by blending commercial xylose-fermenting and wild type Saccharomyces yeast. Bioresource Technology. 282:103-109. https://doi.org/10.1016/j.biortech.2019.02.123.

Economics of seed oil recovery: a review - (Review Article)

The costs of sugar production from different feedstocks and processing technologies - (Review Article)
Singh, V., Dien, B.S., Cheng, M.H., Huang, H. 2019. The costs of sugar production from different feedstocks and processing technologies. Biofuels, Bioproducts, & Biorefining (Biofpr). 13(3):723-739. https://doi.org/10.1002/bbb.1976.

Cellulosic ethanol potential of feedstocks grown on marginal land - (Peer Reviewed Journal)
Kim, S.M, Lee, D., Thapa, S., Dien, B.S., Tumbleson, M.E., Rausch, K.D., Singh, V. 2018. Cellulosic ethanol potential of feedstocks grown on marginal land. American Society of Agricultural and Biological Engineers. 61(6):1775-1782. https://doi.org/10.13031/trans.12945.

Draft genome assemblies for five robust Yarrowia lipolytica strains exhibiting high lipid production and pentose sugar utilization and sugar alcohol secretion from undetoxified lignocellulosic biomass hydrolysates - (Other)
Walker, C., Ryu, S., Quarterman, J.C., Slininger, P.J., Dien, B.S., Trinh, C.T. 2018. Draft genome assemblies for five robust Yarrowia lipolytica strains exhibiting high lipid production and pentose sugar utilization and sugar alcohol secretion from undetoxified lignocellulosic biomass hydrolysates. MICROBIOLOGY RESOURCE ANNOUNCEMENTS. 7(2):e01040-18. https://doi.org/10.1128/MRA.01040-18

Effect of amorphization method on the physicochemical properties of amorphous sucrose - (Peer Reviewed Journal)
Morrow, E.A., Terban, M.W., Thomas, L.C., Gray, D.L., Bowman, M.J., Billinge, S.L., Schmidt, S.J. 2018. Effect of amorphization method on the physicochemical properties of amorphous sucrose. Journal of Food Engineering. 243:125-141. https://doi.org/10.1016/j.jfoodeng.2018.08.036.

Screening for inhibitor tolerant Saccharomyces cerevisiae strains from diverse environments for use as platform strains for production of fuels and chemicals from biomass - (Peer Reviewed Journal)
Mertens, J.A., Kelly, A., Hector, R.E. 2018. Screening for inhibitor tolerant Saccharomyces cerevisiae strains from diverse environments for use as platform strains for production of fuels and chemicals from biomass. Bioresource Technology. 3:154-161. https://doi.org/10.1016/j.biteb.2018.07.006.

Engineering oleaginous yeast strains in the Yarrowia clade for enhanced growth and lipid production from lignocellulosic biomass - (Abstract Only)
Quarterman, J.C., Slininger, P.J., Kurtzman, C.P., Dien, B.S. 2018. Engineering oleaginous yeast strains in the Yarrowia clade for enhanced growth and lipid production from lignocellulosic biomass[abstract]. Symposium on Biotechnology for Fuels and Chemicals. Paper No. M41: Poster No. PS2.

Bioconversion of pelletized big bluestem, switchgrass, and low-diversity grass mixtures into sugars and bioethanol - (Peer Reviewed Journal)
Dien, B.S., Mitchell, R.B., Bowman, M.J., Jin, V.L., Quarterman, J.C., Schmer, M.R., Singh, V., Slininger, P.J. 2018. Bioconversion of pelletized big bluestem, switchgrass, and low-diversity grass mixtures into sugars and bioethanol. Frontiers in Energy Research. 6:129. https://doi.org/10.3389/fenrg.2018.00129.

Fermentation of undetoxified sugarcane bagasse hydrolyzates using a two stage hydrothermal and mechanical refining pretreatment - (Peer Reviewed Journal)
Wang, Z., Dien, B.S., Rausch, K.D,. Tumbleson, M.E., Singh, V. 2018. Fermentation of undetoxified sugarcane bagasse hydrolyzates using a two stage hydrothermal and mechanical refining pretreatment. Bioresource Technology. 261:313-321. https://doi.org/10.1016/j.biortech.2018.04.018.

Absence or presence of metal ion activation in two structurally similar GH43 beta-xylosidases - (Peer Reviewed Journal)
Jordan, D.B., Stoller, J.R., Kibblewhite, R.E., Lee, C.C., Wagschal, K.C. 2018. Analysis of divalent-metal requirements of two GH43 ß-xylosidases by site-directed mutagenesis. Enzyme and Microbial Technology. 114:29-32. doi: 10.1016/j.enzmictec.2018.03.007.

High-conversion hydrolysates and corn sweetener production in dry-grind corn process. - (Peer Reviewed Journal)
Wang, Z., Sharma, V., Dien, B.S., Singh, V. 2018. High-conversion hydrolysates and corn sweetener production in dry-grind corn process. Cereal Chemistry. 95:302-311. https://doi.org/10.1002/cche.10030.

Cultivation and sequencing of rumen microbiome members from the Hungate1000 Collection - (Peer Reviewed Journal)
Seshadri, R., Leahy, S.C., Attwood, G.T., Hoong Teh, K., Lambie, S.C., Eloe-Fadrosh, E.A., Pavlopoulos, G.A, Hadjithomas, M., Varghese, N.J., Paez-Espino, D., Hungate1000 Project Collaborators*: Palevich, N., Janssen,P.H.,Ronimus, R.S., Noel, S., Soni, P., Reilly, K., Atherly, T., Ziemer, C., Wright, A.D., Ishaq, S., Cotta, M., Thompson, S.R., Crosley, K., Mckain, N., Wallace, R.J., Flint, H.J., Martin, J.C., Forster, R.J., Gruninger, R.J., McAllister, T., Gilbert, R., OuwerkerK, D., Klieve, A., Jassim, R.A., Denman, S., McSweeney, C., Rosewarne, C., Koike, S., Kobayashi, Y., Mitsumori, M., Shinkai, T., Cravero, S., Ceron Cucchi, M.*, Perry, R., Henderson, G., Creevey, C.J., Tarrapon, N., Lapebie, P., Drula, E., Lombard, V., Rubin, E., Kyrpides, N.C., Henrissat, B., Woyke, T., Ivanova, N.N, Kelly, W.J. 2018. Cultivation and sequencing of rumen microbiome members from the Hungate1000 Collection. Nature Biotechnology. 36:359-367. doi: 10.1038/nbt.4110.

The impact of stress-response related transcription factors on lignocellulosic hydrolysate inhibitor tolerance of Saccharomyces strains - (Abstract Only)
Mertens, J.A., Hector, R.E., Skory, C.D. 2017. The impact of stress-response related transcription factors on lignocellulosic hydrolysate inhibitor tolerance of Saccharomyces strains [abstract]. Meeting Abstract for Society for Industrial Microbiology, Denver, Colorado, 07/30-08/3/2017. poster #11.

Field productivities of Switchgrass for conversion to sugars and ethanol - (Abstract Only)
Dien, B.S., Bowman, M.J., Casler, M.D., Mitchell, R., Quarterman, J.C., Vogel, K.P. 2017. Field productivities of Switchgrass for conversion to sugars and ethanol [abstract]. Biotechnology for Fuels and Chemicals. Paper No. 14-2.

A general correction to initial rates determined for nonprocessive exo-depolymerases acting on both substrate and product - (Research Notes)
Stoller, J.R., Wagschal, K.C., Lee, C.C., Jordan, D.B. 2017. A general correction to initial rates determined for nonprocessive exo-depolymerases acting on both substrate and product. Analytical Biochemistry. 523:46-49. doi: 10.1016/j.ab.2017.02.004.

Biochemical characterization of a GH43 ß-xylosidase from Bacteroides ovatus - (Peer Reviewed Journal)
Jordan, D.B., Stoller, J.R., Lee, C.C., Chan, V.J., Wagschal, K.C. 2016. Biochemical characterization of a GH43 ß-xylosidase from Bacteroides ovatus. Applied Biochemistry and Biotechnology. 182:250-260. doi: 10.1007/s12010-016-2324-0.

A survey of yeast from the Yarrowia clade for lipid production in dilute-acid pretreated lignocellulosic biomass hydrolysate - (Peer Reviewed Journal)
Quarterman, J., Slininger, P.J., Kurtzman, C.P., Thompson, S.R., Dien, B.S. 2017. A survey of yeast from the Yarrowia clade for lipid production in dilute-acid pretreated lignocellulosic biomass hydrolysate. Applied Microbiology and Biotechnology. 101(8):3319-3334. doi: 10.1007/s00253-016-8062-y.

Maleic acid treatment of biologically detoxified corn stover liquor - (Peer Reviewed Journal)
Kim, D., Ximenes, E.A., Nichols, N.N., Cao, G., Frazer, S.E., Ladisch, M.R. 2016. Maleic acid treatment of biologically detoxified corn stover liquor. Bioresource Technology. 216:437-445. doi: 10.1016/j.biortech.2016.05.086.

Comparative evaluation of 13 yeast species in the Yarrowia clade on lignocellulosic biomass hydrolysate and genetic engineering of inhibitor tolerant strains for lipid and biofuel production - (Abstract Only)
Quarterman, J.C., Slininger, P.J., Kurtzman, C.P., Dien, B.S. 2016. Comparative evaluation of 13 yeast species in the Yarrowia clade on lignocellulosic biomass hydrolysate and genetic engineering of inhibitor tolerant strains for lipid and biofuel production. Meeting Abstract. 231i.

Switchgrass for ethanol and lipid production - (Abstract Only)
Patricia J. Slininger*, Bruce S. Dien, Cletus P. Kurtzman, Bryan R. Moser, Erica L. Bakota, Stephanie R.Thompson, Patricia J. O’Bryan, Michael A. Cotta, Venkatesh Balan, Mingjie Jin, Leonardo D. Sousa, Bruce E. Dale. Process Strategies for High Titers of Lipid Production by Oleaginous Yeasts in Undetoxified Hydrolyzates of Lignocellulosic Biomass. Proceedings of the 2016 AICHE Annual Meeting, Biochemical Conversion Processes in Forest/Plant Biomass Biorefineries Session, Paper # 231i., San Francisco, CA, November 13-18, 2016.

Engineering increased thermostability in the GH-10 endo-1,4-ß-xylanase from Thermoascus aurantiacus CBMAI 756 - (Peer Reviewed Journal)
de Souza, A.R., de Araujo, G.C., Zanphorlin, L.M., Ruller, R., Franco, F.C., Torres, F.A.G., Mertens, J.A., Bowman, M.J., Gomes, E., Da Silva, R. 2016. Engineering increased thermostability in the GH-10 endo-1,4-ß-xylanase from Thermoascus aurantiacus CBMAI 756. International Journal of Biological Macromolecules. 93:20-26. doi: 10.1016/j.ijbiomac.2016.08.056.

Promise of combined hydrothermal/chemical and mechanical refining for pretreatment of woody and herbaceous biomass - (Review Article)
Kim, S.M., Dien, B.S., Singh, V. 2016. Promise of combined hydrothermal/chemical and mechanical refining for pretreatment of woody and herbaceous biomass. Biotechnology for Biofuels. 9(97):1-15. doi: 10.1186/s13086-016-0505-2.

Maleic acid treatment of bioabated corn stover liquors improves cellulose conversion to ethanol - (Abstract Only)
Kim, D., Ximenes, E.A, Nichols, N.N., Cao, G., Frazer, S.E., Ladisch, M.R. 2016. Maleic acid treatment of bioabated corn stover liquors improves cellulose conversion to ethanol [abstract]. Biotechnology for Fuels and Chemicals. M66

Conversion of SPORL pretreated Douglas fir forest residues into microbial lipids with oleaginous yeasts - (Peer Reviewed Journal)
Dien, B.S., Zhu, J.Y., Slininger, P.J., Kurtzman, C.P., Moser, B.R., O'Bryan, P.J., Gleisner, R., Cotta, M.A. 2016. Conversion of SPORL pretreated Douglas fir forest residues into microbial lipids with oleaginous yeasts. RSC Advances. 6(25):20695-20705. doi: 10.1039/c5ra24430g.

Identification of superior lipid producing Lipomyces and Myxozyma yeasts - (Peer Reviewed Journal)
Dien, B.S., Slininger, P.J., Kurtzman, C.P., Moser, B.R., O'Bryan, P.J. 2016. Identification of superior lipid producing Lipomyces and Myxozyma yeasts. AIMS Environmental Science. 3(1):1-20. doi: 10.3934/environsci.2016.1.1.

Kinetic properties of Rhizopus oryzae RPG1 endo-polygalacturonase hydrolyzing galacturonic acid oligomers - (Peer Reviewed Journal)
Mertens, J.A., Bowman, M.J. 2016. Kinetic properties of Rhizopus oryzae RPG1 endo-polygalacturonase hydrolyzing galacturonic acid oligomers. Biocatalysis and Agricultural Biotechnology. 5:11-16. doi: 10.1016/j.bcab.2015.12.005.

Field productivities of Napier grass for production of sugars and ethanol. - (Abstract Only)
Dien, B.S., Anderson, W.F., Lamb, M., O'Bryan, P.J., Slininger, P.J. 2016. Field productivities of Napier grass for production of sugars and ethanol.. Biotechnology for Fuels and Chemicals Symposium Proceedings. 11-2.

Sugar loss and enzyme inhibition due to oligosaccharides accumulation during high solids-loading enzymatic hydrolysis - (Peer Reviewed Journal)
Xue, S., Uppugundla, N., Bowman, M.J., Cavalier, D., Da Cousta Sousa, L., Dale, B.E., Balan, V. 2015. Sugar loss and enzyme inhibition due to oligosaccharides accumulation during high solids-loading enzymatic hydrolysis. Biotechnology for Biofuels. 8:195. doi: 10.1186/s13068-015-0378-9.

Rate-limiting steps of stereochemistry retaining ß-D-xylosidase from Geobacillus stearothermophilus acting as substrates - (Peer Reviewed Journal)
Jordan, D.B., Braker, J.D. 2015. Rate-limiting steps of stereochemistry retaining ß-d-xylosidase from Geobacillus stearothermophilus acting on four substrates. Archives of Biochemistry and Biophysics. 583:73-78. doi: 10.1016/j.abb.2015.08.004.

Miscanthus x giganteus xylooligosaccharides: Purification andfermentation - (Peer Reviewed Journal)
Chen, M.H., Bowman, M.J., Cotta, M.A., Dien, B.S., Iten, L.B., Whitehead, T.R., Rausch, K.D., Tumbleson, M.E., Singh, V. 2016. Miscanthus x giganteus xylooligosaccharides: Purification and fermentation. Carbohydrate Polymers. 140:96-103. doi: 10.1016/j.carbpol.2015.12.052.

Mass spectrometric analysis to identify arabino-xylo-oligomers generated from hydrothermal processing of switchgrass - (Abstract Only)
Bowman, M.J., Nguyen, V.H., Dien, B.S. 2015. Mass spectrometric analysis to identify arabino-xylo-oligomers generated from hydrothermal processing of switchgrass [abstract]. p. 1.

Understanding sugar yield loss and enzyme inhibition due to oligosaccharides accumulation during high solids-loading enzymatic hydrolisis. - (Abstract Only)
Xue, S., Uppugundla, N., Bowman, M.J., Jin, M., da Costa Sousa, L., Chundawat, S.P.S., Fox, B., Cavalier, D., Dale, B.E., Balan, V. 2015. Understanding sugar yield loss and enzyme inhibition due to oligosaccharides accumulation during high solids-loading enzymatic hydrolysis [abstract]. Symposium on Biotechnology for Fuels and Chemicals.

Bioabatement with xylanase supplementation to reduce enzymatic hydrolysis inhibitors - (Abstract Only)
Cao, G., Ximenes, E., Nichols, N.N., Frazer, S.E., Kim, D., Cotta, M.A., Ladisch, M.R. 2015. Bioabatement with xylanase supplementation to reduce enzymatic hydrolysis inhibitors [abstract]. Symposium on Biotechnology for Fuels and Chemicals.

Using isolated cell wall xylan to identify recalcitrant oligosaccharides - (Abstract Only)
Bowman, M.J., Dien, B.S., Mertens, J.A. 2015. Using isolated cell wall xylan to identify recalcitrant oligosaccharides [abstract]. Meeting Abstract. poster session 1

Effect of pelletizing herbaceous grasses on chemical composition and conversion properties - (Abstract Only)
Dien, B.S., Mitchell, R.B., Boateng, A.A., Bowman, M.J., Cotta, M.A., Serapiglia, M., Singh, V. 2015. Effect of pelletizing herbaceous grasses on chemical composition and conversion properties [abstract]. Meeting Abstract. poster session 1.

Bioabatement with hemicellulase supplementation to reduce enzymatic hydrolysis inhibitors - (Peer Reviewed Journal)
Cao, G., Ximenes, E., Nichols, N.N., Frazer, S.E., Kim, D., Cotta, M.A., Ladisch, M. 2015. Bioabatement with hemicellulase supplementation to reduce enzymatic hydrolysis inhibitors. Bioresource Technology. 190:412-415.

Triacetic acid lactone production in industrial Saccharomyces yeast strains - (Peer Reviewed Journal)
Saunders, L.P., Bowman, M.J., Mertens, J.A., Da Silva, N.A., Hector, R.E. 2015. Triacetic acid lactone production in industrial Saccharomyces yeast strains. Journal of Industrial Microbiology and Biotechnology. 42:711-721.

Isolation and characterization of unhydrolyzed oligosaccharides from switchgrass (Panicum virgatum, L.) xylan after exhaustive enzymatic treatment with commercial enzyme preparations - (Peer Reviewed Journal)
Bowman, M.J., Dien, B.S., Vermillion, K.E., Mertens, J.A. 2015. Isolation and characterization of unhydrolyzed oligosaccharides from switchgrass (Panicum virgatum, L.) xylan after exhaustive enzymatic treatment with commercial enzyme preparations. Carbohydrate Research. 407:42-50.