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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Animal Genomics and Improvement Laboratory » Research » Research Project #426992

Research Project: Characterization of DNA Methylome in Livestock Genomes

Location: Animal Genomics and Improvement Laboratory

Project Number: 8042-31000-001-03-A
Project Type: Cooperative Agreement

Start Date: Jul 1, 2014
End Date: Jul 31, 2018

Objective:
To profile and compare the sperm methylomes of bulls that have high and low extreme sire conception rates (SCRs) 1) using bisulfite sequencing (BS-seq); after experimental validations; 2) study functional impacts of differentially methylated regions (DMRs); 3) identify functional DMRs associated with sire conception rates; and 4) develop high-throughput DNA methylation assays using the bisulfite padlock probes (BSPPs) sequencing protocol.

Approach:
ARS will generate over 20 representative sperm DNA methylomes of cattle with high or low male fertility traits using the bisulfite sequencing method MethylC-seq. After experimental validations, we will study functional impacts of differentially methylated regions (DMRs) on genomic features including genes, control elements and common repeats. We will further identify functional DMRs associated with male fertility traits and develop high-throughput DNA methylation assays using the bisulfite padlock probes (BSPPs) sequencing protocol.