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ARS Home » Plains Area » Kerrville, Texas » Knipling-Bushland U.S. Livestock Insects Research Laboratory » LAPRU » Research » Research Project #426918

Research Project: Horn Fly Antennal and Midgut Transcriptome Analysis

Location: Livestock Arthropod Pests Research

Project Number: 3094-32000-038-24-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Sep 1, 2014
End Date: Aug 31, 2019

Objective:
Utilize RNA-Seq technology to develop horn fly tissue-specific transcriptome databases. The sequence information obtained will allow for identification of genes differentially expressed between selected developmental stages and ultimately advance our understanding of pathways critical to horn fly olfaction and insect-microbe interactions.

Approach:
Construct ten TruSeq RNASeq libraries using biological replicates of five different horn fly tissues. The samples will undergo 2x100 base pair paired-end sequencing on a HiSeq2000 instrument, the data of which will be analyzed using bioinformatic tools to assemble de novo horn fly transcriptomes and to conduct specific gene expression profiling between selected experimental conditions.