Location: Subtropical Plant Pathology Research
Project Number: 6034-22000-039-51-A
Project Type: General Cooperative Agreement
Start Date: May 1, 2014
End Date: Apr 15, 2016
1. Characterize Pr-D (FP3) and its role and disease suppression. 2. Investigate the dynamics of the prophages/phages in Las bacteria by revealing the variations in gene expression and recombination. 3. Identify critical elements, such as heat and chemical stress that facilitates lytic activities of the prophages.
1) Identification and characterization of type D phage/prophage in Las bacteria via purification of phage particles by ultracentrifugation, rolling circle amplification, and cloning; 2) RT-PCR analyses of differential gene expression of selected prophage genes in various Las-infected hosts, especially with and without population D; 3) Investigation of stress effect (chemical and physical conditions) on the genes involved in the phage lytic cycle using qPCR.