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ARS Home » Southeast Area » Fort Pierce, Florida » U.S. Horticultural Research Laboratory » Subtropical Plant Pathology Research » Research » Research Project #426811

Research Project: Characterization and Manipulation of the Prophages/phages of 'Candidatus Liberibacter asiaticus' for the Control of Citrus Huanglongbing

Location: Subtropical Plant Pathology Research

Project Number: 6034-22000-039-51-A
Project Type: Cooperative Agreement

Start Date: May 1, 2014
End Date: Apr 15, 2016

Objective:
1. Characterize Pr-D (FP3) and its role and disease suppression. 2. Investigate the dynamics of the prophages/phages in Las bacteria by revealing the variations in gene expression and recombination. 3. Identify critical elements, such as heat and chemical stress that facilitates lytic activities of the prophages.

Approach:
1) Identification and characterization of type D phage/prophage in Las bacteria via purification of phage particles by ultracentrifugation, rolling circle amplification, and cloning; 2) RT-PCR analyses of differential gene expression of selected prophage genes in various Las-infected hosts, especially with and without population D; 3) Investigation of stress effect (chemical and physical conditions) on the genes involved in the phage lytic cycle using qPCR.