Location: Subtropical Plant Pathology Research
Project Number: 6034-22000-042-12-R
Project Type: Reimbursable Cooperative Agreement
Start Date: May 1, 2014
End Date: Apr 30, 2017
1. Characterize Pr-D (FP3) and its role and disease suppression. 2. Investigate the dynamics of the prophages/phages in Las bacteria by revealing the variations in gene expression and recombination. 3. Identify critical elements, such as heat and chemical stress that facilitates lytic activities of the prophages.
This project will be accomplished by: 1) identification and characterization of type D phage/prophage in Las bacteria via purification of phage particles by ultracentrifugation, rolling circle amplification, and cloning; 2) Gene expression analyses for hypothetical protein genes encoded in the prophage regions; 3) Investigation of stress effect (chemical and physical conditions) on the genes involved in the phage lytic cycle; and 4)Improvement of Las culture using Las inoculum with single or incomplete prophage/phage.