Location: Screwworm Research
Project Number: 3094-32000-040-05-I
Project Type: Interagency Reimbursable Agreement
Start Date: May 1, 2014
End Date: Dec 31, 2014
Continue effort to develop a male only strain of screwworm by applying transformation protocols previously developed while also expanding to incorporate approaches successful with Lucilia cuprina.
The project has advanced to the stage where evaluations of existing female conditional lethal vectors have been initiated in the Screwworm Research Unit's facility at Pacora, Panama: this work will continue. We will also develop a second vector system as a contingency in the event the current vectors do not meet the Eradication Program's needs. We will: 1. Develop tetracycline repressible female lethal conditional lethal cloning vector based on improved piggybac transposable element/helper system, C. hominivorax transformer sex-specific splicing intron, tTAV coding region, and tetO operon/promoter. 2. Evaluate the new vector through transformation experiments in the secondary screwworm Cochliomyia macellaria before transferring to C. hominivorax (in Panama).