Location: Foreign Disease-weed Science Research
Project Number: 8044-22000-044-01-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Jun 1, 2014
End Date: Nov 30, 2017
Develop a RNA-based massively parallel sequencing (next generation sequencing) approach for the detection of eukaryotic plant pathogens.
1. Develop an effective universal RNA extraction approach for plants. 2. Apply next generation sequencing techniques to the RNA extracted from pathogen infected plants. 3. Adapt existing bioinformatic tools to utilize next generation RNAseq data for diagnosis of eukaryotic plant pathogens.