Location: Corn Insects and Crop Genetics Research
Project Number: 5030-21220-005-37-R
Project Type: Reimbursable Cooperative Agreement
Start Date: Apr 9, 2014
End Date: Dec 30, 2014
1. Identify the master regulator(s) of iron deficiency response in soybean; 2. Determine if the genetic pathways controlling the iron stress response overlap with other nutrient stress responses; 3. Develop molecular markers for the genes identified in objectives 1 and 2 that can be used for marker assisted selection by breeders to improve soybean agronomic performance
1. Use virus induced gene silencing (VIGS) to turn off candidate genes in the iron stress response. If silencing results in improvment or worsening of IDC symptoms use RNA-seq to characterize the response; 2. Test VIGS constructs developed under objective 1 for involvement in other nutrient stress responses (Nitrogen, Phosphate, Calcium, Sodium) using hydroponics; 3. Once genes improving nutrient stress responses are confirmed, we will use sequence differences between genotypes to develop molecular markers that could be broadly used across soybean cultivars.