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ARS Home » Plains Area » Lincoln, Nebraska » Wheat, Sorghum and Forage Research » Research » Research Project #426424

Research Project: Examine the Role of Wheat Streak Mosaic Virus and Triticum Mosaic Virus Determinants in Mite Transmission and Disease Development in Wheat

Location: Wheat, Sorghum and Forage Research

Project Number: 3042-21000-033-09-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Aug 1, 2014
End Date: Jul 31, 2019

Triticum mosaic virus (TriMV) and Wheat streak mosaic virus (WSMV) are economically important viruses impacting wheat production in the Great Plains region and are transmitted by the wheat curl mites. TriMV determinants responsible for mite transmission are not known. Previously, we found that HC-Pro of WSMV is a viral determinant involved in mite transmission of WSMV. The role of other WSMV proteins in mite transmission has not been examined, and it is possible that more than one viral protein might be involved. Viral coat proteins (CP) play multiple roles in the virus life-cycle and in disease development. Recently, we found that WSMV CP can tolerate extensive deletions and roles of these deletion mutants and TriMV CP are not known in disease development. Specific objectives: 1. Identify and characterize Wheat streak mosaic virus and Triticum mosaic virus protein determinants responsible for transmission by the wheat curl mite. 2. Examine the roles of coat proteins of Wheat streak mosaic virus and Triticum mosaic virus in disease development.

The role of Triticum mosaic virus (TriMV) and Wheat streak mosaic virus (WSMV) proteins in mite transmission will be examined by creating deletion/point mutations into infectious cDNA clones of WSMV and TriMV. Wheat plants infected with mutant viruses will be used as the virus source for acquisition of virus by aviruliferous mites. After a virus acquisition feeding period, mites will be transferred to test plants to determine the effects of mutations on mite transmission. Deletion and/or point mutations will be introduced into the coat protein encoding genes of WSMV and TriMV, and examined to establish the effects of mutations on virus’s ability to infect wheat systemically. Viable mutants will be examined for the effects of disease development in wheat and other host plants, and will also be examined for the role of coat protein in mite transmission, cell-to-cell and long-distance movement in wheat.