Project Number: 8040-52000-063-16-I
Project Type: Interagency Reimbursable Agreement
Start Date: Oct 1, 2013
End Date: Sep 30, 2014
1. Acquire authentic raw plants and commercially available supplements for challenging botanical materials. 2. Develop spectral and chromatographic fingerprint methods using NIR, UV, MS, NMR, HPLC, and UHPLC for rapid identification based on chemical composition. 3. Acquire DNA barcodes for selected raw and processed botanical supplements. 4. Acquire metabolite profiles using UHPLC- MS and high field 1H-NMR. 5. Acquire DNA barcodes for raw and, where possible, commercial botanical materials. 6. Develop a prototype database for botanical supplements with taxonomic, genetic, fingerprint, and metabolomic profiles.
New and innovative methods will be used to identify, authenticate, and characterize botanical supplements - from raw ingredients to processed products. Development of these methods is challenging to the analyst and necessary for the consumer and industry. Over the next five years, in order to provide the clearest possible description of botanical supplements, their components, and their variability, analytical data with taxonomic and genetic data will be combined. Obtain a wide range of authentic raw materials through purchases and collaborations to account for expected natural variation. Acquire, through collaboration, a national sampling of commercially available botanical supplements. Investigate, through an SCA with the North Carolina Arboretum, a variety of challenging botanicals that are of commercial interest (Aloe sp. and Camellia sinensis (L.) Kuntze -tea) or exhibit altered phenotypes as a result of hybridization (Echinacea sp.) or endophytic fungi (Actaea racemosa L., black cohosh). Use chemical fingerprinting, based on both chromatographic and direct spectral analysis, and chemometric data processing to provide rapid identification of botanicals and to discriminate between authentic and adulterated materials. Use ultra high performance liquid chromatography-high resolution accurate mass/mass spectrometry (UHPLC-HRMS) and high field proton nuclear magnetic resonance (1H-NMR) spectrometry (through an RSA with the University of Maryland) to develop metabolite profiles of the raw botanical materials. Acquire DNA barcodes for all the materials analyzed through an SCA with AuthenTechnologies. Combine these data (taxonomic, genetic, fingerprint, and metabolomic profiles) in a prototype database that, when finalized, will allow identification of raw botanicals through DNA barcodes or chemical fingerprints and will provide access to the full chemical composition of the botanical.