Research Project: Bioavailability of Iron, Zinc and Select Phytochemicals for Improved Health

Location: Plant, Soil and Nutrition Research

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Seasonal trends of nutrient intake in rainforest communities of northeastern Madagascar - (Peer Reviewed Journal)
Golden, C., Vaitla, B., Ravaoliny, L., Vonona, M., Anjaranirina, E., Randriamady, H., Guth, S., Fernald, L., Myers, S., Glahn, R.P. 2019. Seasonal trends of nutrient intake in rainforest communities of northeastern Madagascar. Public Health Nutrition. 12:2200-2209. https://doi.org/10.1017/S1368980019001083.

Metabolic engineering of bread wheat improves grain iron concentration and bioavailability - (Peer Reviewed Journal)
Beasley, J., Bonneau, J., Sanchez-Palacious, J., Moreno-Moyano, L., Callahan, D., Tako, E.N., Glahn, R.P., Lombi, E., Johnson, A. 2019. Metabolic engineering of bread wheat improves grain iron concentration and bioavailability. Plant Biotechnology Journal. 17(8):1514-1526. https://doi.org/10.1111/pbi.13074.

Iron biofortified carioca bean (phaseolus vulgaris l.) —based Brazilian diet delivers more absorbable iron and affects the gut microbiota in vivo (gallus gallus) - (Peer Reviewed Journal)
Morais Dias, D., Kolba, N.J., Binyamin, D., Ziv, O., Regini Nutti, M., Stampini Duarte, M., Glahn, R.P., Koren, O., Tako, E.N. 2018. Iron biofortified carioca bean (phaseolus vulgaris l.) —based Brazilian diet delivers more absorbable iron and affects the gut microbiota in vivo (gallus gallus). Nutrients. 10(12):1970. https://doi.org/10.3390/nu10121970.

ZnO nanoparticles affect nutrient transport in an in vitro model of the small intestine - (Peer Reviewed Journal)
Tako, E.N., Mahler, G., Olivas, F.M. 2018. ZnO nanoparticles affect nutrient transport in an in vitro model of the small intestine. Food and Chemical Toxicology. https://doi.org/10.1016/j.fct.2018.11.048.

The fast cooking and enhanced iron bioavailability properties of the manteca yellow bean (Phaseolus vulgaris L.) - (Peer Reviewed Journal)
Wiesinger, J.A., Cichy, K.A., Tako, E.N., Glahn, R.P. 2018. The fast cooking and enhanced iron bioavailability properties of the manteca yellow bean (Phaseolus vulgaris L.). Nutrients. 10(11):1609. https://doi.org/10.3390/nu10111609.

Alterations in the gut (Gallus gallus) microbiota following the consumption of zinc biofortified wheat (Triticum aestivum) -based diet - (Peer Reviewed Journal)
Reed, S., Knez, M., Uzan, A., Stangoulis, J., Glahn, R.P., Koren, O., Tako, E.N. 2018. Alterations in the gut (Gallus gallus) microbiota following the consumption of zinc biofortified wheat (Triticum aestivum) -based diet. Journal of Agricultural and Food Chemistry. 66(25):6291-6299. https://doi.org/10.1021/acs.jafc.8b01481.

Development of a fortified juice beverage: assessment of iron bioavailability using an established caco-2 cell bioassay - (Peer Reviewed Journal)
Fleige, L., Bhoite, R., Marwaha, A., Glahn, R.P. 2018. Development of a fortified juice beverage: assessment of iron bioavailability using an established caco-2 cell bioassay. Journal of Nutrition & Food Sciences. 6(3):1-7. https://doi.org/10.15226/jnhfs.2018.001133.

Evaluation of selected ultra-trace minerals in commercially available dry dog foods - (Peer Reviewed Journal)
Wakshlag, J., Glahn, R.P., Kim, H., Loftus, J., Gagne, J., Rutzke, M. 2018. Evaluation of selected ultra-trace minerals in commercially available dry dog foods. Veterinary Medicine: Research and Reports. 9:43-51. https://doi.org/10.2147/VMRR.S165890.

Silicon dioxide nanoparticle exposure affects small intestine function in an in vitro model - (Peer Reviewed Journal)
Guo, Z., Martucci, N., Liu, Y., Mahler, G., Yoo, E., Tako, E.N. 2018. Silicon dioxide nanoparticle exposure affects small intestine function in an in vitro model. Nanotoxicology. 12(5):485-508. https://doi.org/10.1080/17435390.2018.1463407.

The in ovo feeding administration (gallus gallus)-an emerging in vivo approach to assess bioactive compounds with potential nutritional benefits - (Peer Reviewed Journal)
Hou, T., Tako, E.N. 2018. The in ovo feeding administration (gallus gallus)-an emerging in vivo approach to assess bioactive compounds with potential nutritional benefits. Nutrients. 10(4):418. https://doi.org/10.3390/nu10040418.

The linoleic acid: dihomo-y-linolenic acid ratio (LA:DGLA)—an emerging biomarker of Zn status - (Book / Chapter)
Knez, M., Stangoulis, J., Glibetic, M., Tako, E. 2018. The linoleic acid: dihomo-y-linolenic acid ratio (LA:DGLA)—an emerging biomarker of Zn status. In: Tako, E. Dietary Zn and Human Health. Basel, Switzerland: MDPI Books. p. 1-12.

Relative bioavailability of iron in Bangledeshi traditional meals prepared with iron-fortified lentil dal - (Peer Reviewed Journal)
Podder, R., Dellavalle, D., Tyler, R., Glahn, R.P., Tako, E.N., Vandenberg, A. 2018. Relative bioavailability of iron in Bangledeshi traditional meals prepared with iron-fortified lentil dal. Nutrients. 10(3). https://doi.org/10.3390/nu10030354.

The linoleic acid: dihomo-y-linolenic acid ratio predicts the efficacy of Zn biofortified wheat in chicken (Gallus gallus) - (Peer Reviewed Journal)
Tako, E.N., Knez, M., Kolba, N.J., Glahn, R.P., Ireland, E., Stangoulis, J. 2018. The linoleic acid: dihomo-y-linolenic acid ratio predicts the efficacy of Zn biofortified wheat in chicken (Gallus gallus). Journal of Agricultural and Food Chemistry. 66(6):1394-1400. http://doi.org/10.1021/acs.jafc.7b04905.

Iron bioavailability studies of the first generation of iron-biofortified beans released in Rwanda - (Book / Chapter)
Glahn, R.P., Hart, J.J., Beebe, S., Tako, E.N. 2018. Iron bioavailability studies of the first generation of iron-biofortified beans released in Rwanda. In: Tako, E., editor. Fe deficiency, dietary bioavailability and absorption. Basel, Switzerland: MDPI Books. p. 16-27. https://doi.org/10.3390/books978-3-03897-231-0.

Intra amniotic administration of raffinose and stachyose affects the intestinal brush border functionality and alters gut microflora populations - (Book / Chapter)
Tako, E. 2017. Intra amniotic administration of raffinose and stachyose affects the intestinal brush border functionality and alters gut microflora populations. In: McCrory, M., editor. Dietary Fibers and Human Health. Basel, Switzerland: MDPI. p. 48-58. https://doi.org/10.3390/books978-3-03842-582-3.

Physico-chemical properties, phytochemicals and DPPH radical scavenging activity of supercritical fluid extruded lentils - (Peer Reviewed Journal)
Ying, L., Glahn, R.P., Hebb, R., Rizvi, S. 2017. Physico-chemical properties, phytochemicals and DPPH radical scavenging activity of supercritical fluid extruded lentils. LWT - Food Science and Technology. 89:315-321. https://doi.org/10.1016/j.lwt.2017.10.063.

Physico-chemical properties, phytochemicals and antioxidant activity of supercritical fluid extruded lentils - (Peer Reviewed Journal)
Yv, Y., Glahn, R.P., Hebb, R., S.H. Rizvi, S. 2017. Physico-chemical properties, phytochemicals and antioxidant activity of supercritical fluid extruded lentils. LWT - Food Science and Technology. 89:315-321. https://doi.org/10.1016/j.lwt.2017.10.063.

The linoleic acid: dihomo-y-linolenic acid ratio (LA:DGLA)— an emerging biomarker of Zn status - (Peer Reviewed Journal)
Knez, M., Stangoulis, J., Tako, E. 2017. The linoleic acid: dihomo-y-linolenic acid ratio (LA:DGLA)— an emerging biomarker of Zn status. Nutrients. 9(8):825.

Intra-amniotic administration (Gallus gallus) of cicer arietinum and lens culinaris prebiotics extracts and duck egg white peptides affects calcium status and intestinal functionality - (Peer Reviewed Journal)
Hou, T., Kolba, N.J., Glahn, R.P., Tako, E. 2017. Intra-amniotic administration (Gallus gallus) of cicer arietinum and lens culinaris prebiotics extracts and duck egg white peptides affects calcium status and intestinal functionality. Nutrients. 9(7):785. doi:10.3390/nu9070785.

Characterizing the gut (Gallus gallus) microbiota following the consumption of an iron biofortified Rwandan cream seeded carioca (Phaseolus Vulgaris L.) bean-based diet - (Peer Reviewed Journal)
Reed, S., Neuman, H., Glahn, R.P., Omry, K., Tako, E.N. 2017. Characterizing the gut (Gallus gallus) microbiota following the consumption of an iron biofortified Rwandan cream seeded carioca (Phaseolus Vulgaris L.) bean-based diet. PLoS One. 12(8):e0182431. https://doi.org/10.1371/journal.pone.0182431.

Iron bioavailability studies of the first generation of iron-biofortified beans released in Rwanda - (Peer Reviewed Journal)
Glahn, R.P., Tako, E., Hart, J.J., Haas, J., Lung'Aho, M., Beebe, S. 2017. Iron bioavailability studies of the first generation of iron-biofortified beans released in Rwanda. Nutrients. 9(7):787.

Characterization of polyphenol effects on inhibition and promotion or iron update by caco-2 cells - (Peer Reviewed Journal)
Hart, J.J., Tako, E.N., Glahn, R.P. 2017. Characterization of polyphenol effects on inhibition and promotion or iron update by caco-2 cells. Journal of Agricultural and Food Chemistry. 65(16):3285-3294.

Intra amniotic administration of raffinose and stachyose affects the intestinal brush border functionality and alters gut microflora populations - (Peer Reviewed Journal)
Pacifici, S., Song, J., Zhang, C., Wang, Q., Glahn, R.P., Kolba, N.J., Tako, E.N. 2017. Intra amniotic administration of raffinose and stachyose affects the intestinal brush border functionality and alters gut microflora populations. Nutrients. 9(3):304. doi:10.3390/nu9030304.

Advantages and limitations of in vitro and in vivo methods of iron and zinc bioavailability evaluation in the assessment of biofortification program effectiveness - (Peer Reviewed Journal)
Dias, D., Costa, N., Nutti, M., Tako, E.N., Martino, H. 2017. Advantages and limitations of in vitro and in vivo methods of iron and zinc bioavailability evaluation in the assessment of biofortification program effectiveness. Critical Reviews in Food Science and Nutrition. http://doi.org/10.1080/10408398.2017.1306484.

Titanium dioxide nanoparticle ingestion alters nutrient absorption in an in vitro model of the small intestine - (Peer Reviewed Journal)
Guo, Z., Martucci, N., Moreno-Olivas, F., Mahler, G., Tako, E.N. 2017. Titanium dioxide nanoparticle ingestion alters nutrient absorption in an in vitro model of the small intestine. NanoImpact. 5:70-82.

The combined application of the Caco-2 cell bioassay coupled with in vivo (Gallus gallus) feeding trial represents an effective approach to predicting Fe bioavailability in humans - (Peer Reviewed Journal)
Tako, E.N., Bar, H., Glahn, R.P. 2016. The combined application of the Caco-2 cell bioassay coupled with in vivo (Gallus gallus) feeding trial represents an effective approach to predicting Fe bioavailability in humans. Nutrients. 8(11):732.

A novel in vivo model for assessing the impact of geophagic earth on iron status - (Peer Reviewed Journal)
Siem, G., Tako, E.N., Ahn, C., Bodis, M., Glahn, R.P., Young, S. 2016. A novel in vivo model for assessing the impact of geophagic earth on iron status. Nutrients. 8(6):E362. doi:10.3390/nu8060362.

The cotyledon cell wall of the common bean (phaseolus vulgaris) resists digestion in the upper intestine and thus may limit iron bioavailability - (Peer Reviewed Journal)
Glahn, R.P., Tako, E.N., Cichy, K.A., Wiesinger, J.A. 2016. The cotyledon cell wall of the common bean (phaseolus vulgaris) resists digestion in the upper intestine and thus may limit iron bioavailability. Food & Function. 7(7):3193-3200.

Biofortified indica rice attains iron and zinc nutrition dietary targets in the field - (Peer Reviewed Journal)
Trijatmiko, K.R., Duenas, C., Torrizo, L., Oliva, N., Francisco, P., Mae Arines, F., Adeva, C., Balindong, J., Sapasap, V., Barry, G., Slamet-Loedin, I.H., Lombi, E., Stangoulis, J., Johnson, A., Tako, E.N., Glahn, R.P. 2016. Biofortified indica rice attains iron and zinc nutrition dietary targets in the field. Scientific Reports. 6:19792.

Chronic zinc deficiency alters chick gut microbiota composition and function - (Peer Reviewed Journal)
Reed, S.M., Neuman, H., Moskovitch, S., Glahn, R.P., Koren, O., Tako, E.N. 2015. Chronic zinc deficiency alters chick gut microbiota composition and function. Nutrients. 7:1-17.

Iron bioavailability in low phytate pea - (Peer Reviewed Journal)
Liu, X., Glahn, R.P., Arganosa, G.C., Warkentin, T.D. 2014. Iron bioavailability in low phytate pea. Crop Science. 55(1):320-330.

Extrinsic labelling of staple food crops with isotopic iron does not consistently result in full equilibration: Revisiting the methodology - (Peer Reviewed Journal)
Glahn, R.P., Cheng, Z., Giri, S. 2015. Extrinsic labelling of staple food crops with isotopic iron does not consistently result in full equilibration: Revisiting the methodology. Journal of Agricultural and Food Chemistry. 63(43):9621-9628.

Studies of cream seeded carioca beans (phaseolus vulgaris L.) from a Rwandan efficacy trial: in vitro and in vivo screening tools reflect human studies and predict beneficial results from iron biofortified beans - (Peer Reviewed Journal)
Tako, E.N., Reed, S.M., Anandaraman, A., Hart, J.J., Beebe, S.E., Glahn, R.P. 2015. Studies of cream seeded carioca beans (phaseolus vulgaris L.) from a Rwandan efficacy trial: in vitro and in vivo screening tools reflect human studies and predict beneficial results from iron biofortified beans. PLoS One. DOI: 10.1371/journal.pone.0138479.

Iron absorption from intrinsically-labeled lentils - (Peer Reviewed Journal)
Della Valle, D.M., Glahn, R.P., Shaff, J., O'Brien, K.O. 2015. Iron absorption from intrinsically-labeled lentils. Journal of Nutrition. DOI: 10.3945/jn.115.217273.

Higher iron pearl millet (Pennisetum glaucum L.) provides more absorbable iron that is limited by increased polyphenolic content - (Peer Reviewed Journal)
Tako, E.N., Reed, S.M., Budiman, J., Hart, J.J., Glahn, R.P. 2015. Higher iron pearl millet (Pennisetum glaucum L.) provides more absorbable iron that is limited by increased polyphenolic content. Nutrition Journal. 14:11.

Alterations in gut microflora populations and brush border functionality following intra-amniotic daidzein administration - (Peer Reviewed Journal)
Hortono, K., Reed, S.M., Ankrah, A., Glahn, R.P., Tako, E.N. 2014. Alterations in gut microflora populations and brush border functionality following intra-amniotic daidzein administration. RSC Advances. 5:6407-6412.

Differences in relative bioavailability of traditional Bangladeshi meal plans - (Peer Reviewed Journal)
Dellavalle, D.M., Glahn, R.P. 2014. Differences in relative bioavailability of traditional Bangladeshi meal plans. Food and Nutrition Bulletin. 35(4):431-439.

The effect of wheat prebiotics on the gut bacterial population and iron status of iron deficient broiler chickens - (Peer Reviewed Journal)
Tako, E.N., Marija, K., Glahn, R.P., Stangoulis, J. 2014. The effect of wheat prebiotics on the gut bacterial population and iron status of iron deficient broiler chickens. Nutrition Journal. 13:58.

Polyphenolic compounds appear to limit the nutritional benefit of biofortified higher iron black bean (phaseolus vulgaris L.) - (Peer Reviewed Journal)
Tako, E.N., Beebe, S.E., Hart, J.J., Reed, S.M., Glahn, R.P. 2014. Polyphenolic compounds appear to limit the nutritional benefit of biofortified higher iron black bean (phaseolus vulgaris L.). Nutrition Journal. 13:28.