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ARS Home » Pacific West Area » Pullman, Washington » Grain Legume Genetics Physiology Research » Research » Research Project #426304

Research Project: Multiplication and Screening Common Bean Lines for Distribution and Evaluation of Disease Response

Location: Grain Legume Genetics Physiology Research

Project Number: 2090-21220-002-03-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Jan 1, 2014
End Date: Dec 31, 2018

The objectives of this project are twofold: 1) multiply seed of large populations (panels of accessions for association mapping) for worldwide distribution but primarily countries in East Africa, and 2) evaluate the phenotypic response of the same populations to various diseases in South Africa including fungal - Angular Leaf Spot, Bean Rust, and Halo Bacterial Blight, for GWAS (genome wide association studies) and for parental selection of resistant accessions for use in bean breeding programs.

Small seed samples of accessions of common bean will be obtained and complied into panels of accessions for association mapping studies for biotic and abiotic stress resistance traits. Approximately 200 seeds of each accession will be sown in two row plots of 5 m lengths for seed increase. The increased seed of the panels will be organized in planting envelopes with specific seed numbers for different repicated experiments in South Africa and other countries. Seed will be grown under disease-free condition and will be inspected for diseases and pests periodically by a governmental seed certifying agency. In South Africa, once seed is increased, the panels of accessions will be planted in randomized complete blocks in lattice designs with two to three replications to evaluate response to different diseases. Spreader rows of common bean lines susceptible to the targeted disease will be inoculated and the irrigation managed to promote uniform spread of the disease for each trial. Each plot repesenting one replicate of an accession will be rated for level of disease multiple times during the growing season (trials will comprise ~1200 plots, thus it may take multiple days for a single rating).