Project Number: 8042-21220-180-00-D
Project Type: In-House Appropriated
Start Date: Dec 27, 2013
End Date: Jun 6, 2017
Objective 1) Develop an alternative, sustainable management strategy for soilborne diseases of cucumber. Sub-objective 1A: Develop multitactic disease control strategies effective against important soilborne plant pathogens of cucumber and other cucurbits that are effective over a range of soil conditions. Biologically based technologies (beneficial bacteria and fungi, microbial extracts, and cover crops) will be screened for suppression of the soilborne pathogens Pythium ultimum, Rhizoctonia solani, and Meloidogyne incognita on cucumber and other cucurbits. The best performing biologically based technologies will be combined and the performance of these combined technologies assessed in various soils. Approaches will be developed and used to enhance the performance of combined technologies. Sub-objective 1B: Identify traits in Pseudomonas protegens strain Pf-5 which allow biological control agents to adapt to environmental factors in plant spermosphere and rhizosphere. The role of the regulatory proteins Vfr and Crc in colonization and suppression of P. ultimum and R. solani on cucumber will be determined. Genes regulated by Vfr and Crc and the role that these regulated genes have in colonization and disease suppression will be determined. Objective 2) Development of molecular tools to detect, identify, characterize, and mitigate the deleterious effects of soil-borne pathogens, including Rhizoctonia solani, in ornamentals, vegetables, and turf grasses. [NP303, C4, PS4C]
Basic microbiology techniques to develop biologically based technologies that are effective in suppression of important soilborne plant pathogens of cucumbers and other cucurbits will be used. Multi-disciplinary collaboration in growth chamber, greenhouse, and field studies will evaluate the effectiveness of these biologically based technologies in a variety of soil types. Molecular and proteomic approaches will be applied to determine the role of the regulatory proteins Vfr and Crc in colonization and suppression of P. ultimum and R. solani on cucumber. Proteomic and gene knock-out approaches will be used to determine the genes regulated by Vfr and Crc and the role that these regulated genes have in colonization and disease suppression.