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ARS Home » Southeast Area » Fort Pierce, Florida » U.S. Horticultural Research Laboratory » Subtropical Insects and Horticulture Research » Research » Research Project #426032

Research Project: Identification of Bacillius Thuringiensis EndoToxins Active Against Adult Asian Citrus Psyllid

Location: Subtropical Insects and Horticulture Research

Project Number: 6034-22320-004-09-R
Project Type: Reimbursable Cooperative Agreement

Start Date: Nov 14, 2013
End Date: Apr 30, 2017

The objective of this project is to screen strains of the bacterium Bacillus thuringiensis (Bt), for activity against the Asian citrus psyllid (ACP) and to identify ACP-active crystal (Cry) toxins.

We will screen at least 200 Bt strains for toxicity against Asian citrus psyllid (ACP). Total crystal proteins from these strains will be purified, activated using bovine trypsin and tested for ACP toxicity. Individual Cry toxins from ACP-toxic Bt strains will be purified to homogeneity by ion-exchange and gel filtration column chromatography. These individual toxins will then be tested for ACP toxicity and identified by MALDI-TOF MS/MS.