Project Number: 3040-32000-031-04-R
Project Type: Reimbursable Cooperative Agreement
Start Date: Aug 15, 2013
End Date: May 15, 2014
To describe the epidemiology of Mannheimia haemolytica and other relevant respiratory disease pathogens in calves after comingling. To evaluate the effect of treatment for control of BRD on the spread of M. haem and dominance of isolates in the group as compared to groups not receiving control treatment.
Nasopharyngeal cattle samples will be collected from a single group of commercial cattle at up to six time points, at the sale barn and the backgrounding facility. These samples, collected from the same individuals, will be analyzed at USMARC with classical microbiological techniques for pathogen isolation and characterization, as well as with genomic and metagenomic approaches. A subset of the pathogens isolated will be characterized using biochemical assays (Biolog) as well as whole genome sequencing (MiSeq/PacBio RS). The nasopharyngeal samples will also be analyzed using metagenomic approaches. Using these combinations of technologies we will detect changes in the microbial flora in the nasopharynx of individual cattle as they move though the production system and are subjected to antimicrobial treatments.