Location: Peanut Research
Project Number: 6044-21000-004-03-S
Project Type: Specific Cooperative Agreement
Start Date: May 1, 2014
End Date: Jun 30, 2017
Identify peanut genotypes with elevated folate levels in seeds, compared to publically-released peanut varieties, through the evaluation of transformed peanuts utilizing a newly developed gene-transfer method and novel genetic lines through traditional breeding.
Improved germplasm from ongoing ARS biotechnology and breeding programs will be grown in greenhouse, small sub-plots in the field, or under environmental controlled small plots. Mature peanut seeds will be collected at the end of the growing season and treated according to industry standards. Individual seeds or pooled seeds of the same peanut genotype will be subjected to folate analysis utilizing microbiologic assay and HPLC/MS analysis. Results will be subjected to statistical analysis. Seeds with elevated folate levels will be utilized in peanut breeding program.