Project Number: 8042-31000-103-09-T
Project Type: Trust Fund Cooperative Agreement
Start Date: Sep 1, 2013
End Date: Aug 31, 2016
Use site-directed genetic modification of the NANOS3 gene, via genome editing, to produce livestock (pigs) animals lacking spermatogonial stem cells (SSCs). The testes of modified males are expected to lack endogenous spermatogenesis, but provide a competent environment to support spermatogenesis initiated from transplanted genetically elite or engineered (cultured) SSCs.
The Sponsor will provide transcription activator-like effector nucleases (TALENs) that have been validated in vitro for the ability to target and modify the NANOS3 gene. UMD-CP and ARS scientists will use these enzymes to disrupt the NANOS3 gene by site directed mutagenesis. UMD and ARS scientists will inject TALENs into zygotes which will then be transferred into recipient females Offspring will be screened for genetic alterations in the NANOS3 gene expected to result in SSC deficient males. The animals will be housed and reared in ARS facilities. ARS, UMDCP & the Cooperator will evaluate all data and publish jointly.