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ARS Home » Northeast Area » Beltsville, Maryland (BHNRC) » Beltsville Human Nutrition Research Center » Food Components and Health Laboratory » Research » Research Project #425569

Research Project: Adaptation in Polyphenol Bioavailability and Bioactivity During Long Term Exposure to Polyphenol-Rich Foods in Lean And Obese Individuals

Location: Food Components and Health Laboratory

Project Number: 8040-51000-056-07-I
Project Type: Interagency Reimbursable Agreement

Start Date: Apr 1, 2014
End Date: Mar 31, 2018

Objective:
The goals of this project are to assess differential response in bioavailability and metabolic pathways between lean and obese individuals exposed to acute, short term (10-day), and long-term (10-week) consumption of polyphenol-rich fruits (blueberry and apple) and beverages (tea); determine the differential systemic and gut anti-inflammatory response between obese and lean individuals exposed to polyphenol-rich fruits (blueberry and apple) and beverages (tea); and identify mechanisms related to adaptation in polyphenol absorption, metabolism, and modulation of inflammatory processes in the GI tract.

Approach:
A study will be conducted in which lean and obese volunteers will consume polyphenol-rich blueberries (anthocyanins, flavonols), apples (flavan-3-ols) and tea (flavan-3-ols) for 10 weeks. On three occasions (at the beginning, after 2 weeks, and after 10 weeks of the intervention), a bolus dose of polyphenol-rich berries and tea will be provided in the morning followed by serial blood and urine collections and a fecal collection. Polyphenol metabolites will be measured in blood and urine by LC-MS/MS for mathematical modeling. Cytokines and acute phase proteins will be measured in fecal water and serum. Expression of genes related to pathways of inflammation will be assessed by real time RT-PCR in peripheral blood mononuclear cells. In parallel, a cell-based model (Caco-2) will be used to investigate mechanisms associated with adaptation of polyphenol absorption and metabolism over of long-term treatment.