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ARS Home » Northeast Area » Orient Point, New York » Plum Island Animal Disease Center » Foreign Animal Disease Research » Research » Research Project #425515

Research Project: Simple Diagnostic Tools for Rapid Decision Making in Feedlots: Development of Multiplex RT-LAMP Assay to Discriminate Between FMDV and VS Isolates

Location: Foreign Animal Disease Research

Project Number: 8064-32000-057-56-N
Project Type: Non-Funded Cooperative Agreement

Start Date: Sep 1, 2013
End Date: Dec 31, 2014

Foot-and-Mouth Disease Virus (FMDV) and Vesicular Stomatitis viruses (VSVs) display similar clinical signs in infected livestock including lesions, vesicles and fever. Currently there are no diagnostic tools available which can distinguish between the diseases. This research project seeks to develop a simple to use diagnostic test that can be applied to suspect cases of Foot-nd-Mouth Disease (FMD) and Vesicular Stomatitits (VS) in the United States through the use of multiplex isothermal loop amplification assays and a molecular lateral flow device.

A Real-Time Loop Mediated Isothermal Amplifcation (RT-LAMP) assay will be developed for the detection of VSVs as a companion test for the existing RT-LAMP assays that are already available for FMDV. Using representative VSVs strains the performance of this test will be validated and compared to established laboratory methods used for VS diagnosis. The new LAMP test will then be combined in a multiplex format with a RT-LAMP for the detection of FMDV allowing simultaneous discrimination between thse two important viral agents. In addition, these assays will be transferred onto a simple lateral-flow device (LFD) readout format for use in laboratory instrumentation, such as PCR machines. This new test will provide unambiguous results utilizing customized LFDs that can distinguish between dual labeled products generated by the FMDV and VSVs RT-LAMP assays, respectively.