Location: Vegetable Crops Research
Project Number: 5090-21220-003-00-D
Project Type: In-House Appropriated
Start Date: Oct 1, 2013
End Date: Mar 6, 2018
Objective 1: Develop and apply genomic and genetic tools to map and characterize the genetic bases of the key cranberry traits that determine yield. Objective 2: Based on horticultural, genetic, and genomic information, formulate and apply breeding approaches for genetically improving cranberry yield. Objective 3: Determine the development thresholds of key arthropod pests (cranberry fruitworm and Sparganothis fruitworm) to better predict the developmental status of populations in the field. Objective 4: Develop novel, innovative IPM strategies to reduce pesticide use and sustain cranberry yield, quality, and profitability. Objective 5: Develop alternative cranberry production practices that improve water conservation and decrease plant disease.
Objective 1: Next-generation sequencing technology will be used to characterize the cranberry genome. The resultant data will be used to discover and mine molecular markers such as SSRs and SNPs. We will then develop high-resolution genetic maps using the developed markers based on several available half-sib F1 mapping populations. Phenotyping will involve collecting data on yield-related traits and other horticultural measurements, including total fruit weight, percent rotten fruit, average berry weight, and fruit quality parameters. These traits will be localized in the linkage map described above. Information derived from the high resolution cranberry linkage map with yield-related will be used to plan strategic crosses. Objective 2: Prior to creating cranberry hybrids, horticultural, genetic, and genomic information will be carefully considered to ensure that strategic crosses are accomplished. A microsatellite marker based fingerprinting assay will be developed for the true-to-type verification of the cranberry cultivars. We will also characterize known cranberry diversity from the breeding programs and collections and samples sent in by growers. Pedigree information will be evaluated in the light of marker information to determine the most likely genotypes or genetic pools associated with each named cultivar and their associated horticultural performance. A series of cranberry hybrids with complementary genetic pools will be created and evaluated. Objective 3: The temperature-specific development rates and degree-day (DD) accumulations associated with cranberry fruitworm (CFW) and Sparganothis fruitworm (SFW) will be determined. Larval growth rates will be measured over a wide range of controlled temperatures. Growth rates will be plotted against temperature, and models will be fit to the dynamic. From these models, the lower and upper development thresholds will be isolated. The thresholds will then be used to generate degree-day (DD) accumulations that can be linked to discrete biological events, such as flight initiation in the field, adult lifespan, ovipositional period, and egg-hatch periods. DD accumulations represent key developmental benchmarks, helping to optimize pest management in the cranberry system. Objective 4: novel insect pest management approaches will be investigated. Two primary tactics will be explored within the cranberry system: pheromone-based mating disruption and trophic position measurement. In partnership with private industry, as well as Wisconsin cranberry growers, the first ever 3-species mating disruption program will be deployed at large scales within commercial marshes. Population suppression of the target pests will be assayed and compared with conventional pest management approaches. Studies of arthropod trophic position will be conducted using stable isotopic analysis of amino acids. Trophic position estimation will reveal the lifetime trophic tendencies of carnivorous species, thereby providing empirical evidence as to which species are actually beneficial for cranberry production.