Location: Foreign Animal Disease Research
Project Number: 8064-32000-060-03-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Aug 1, 2013
End Date: Jul 31, 2018
The African Swine Fever Virus (ASFV) genome encodes for more than 50 polypeptides and most of these genes have not been properly characterized in terms of their involvement in virus virulence and interaction with host proteins. The study of the interaction between these viral genes and the host is critical in the understanding the mechanism causing virus attenuation during infection. This research project seeks to analyze and characterize the host proteins which interact with viral proteins during infection.
Specific host proteins which interact with viral proteins will be identified by USDA, ARS using a yeast two hybrid system. The proteins which demonstrate positive interaction will be further analyzed by INIA in ASFV infected cells using different methodologies including; coimmunoprecipitation, mammalian two hybrid system and confocal immune fluorescence. These methodologies will provide further characterization of the virus-host protein interactions. The cellular metabolic pathways involved in these interactions will be analyzed in order to gain an understanding of the possible role of the host-virus interaction.