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ARS Home » Pacific West Area » Maricopa, Arizona » U.S. Arid Land Agricultural Research Center » Pest Management and Biocontrol Research » Research » Research Project #425294

Research Project: Rapid Quantification of Aflasafe-Fungi on Crops in Africa to Verify Proper Application of Aflasafe

Location: Pest Management and Biocontrol Research

Project Number: 2020-42000-022-13-T
Project Type: Trust Fund Cooperative Agreement

Start Date: Jan 15, 2014
End Date: Dec 31, 2018

Objective:
Perform research on development of specific assays to quantify usage of aflatoxin biological control agents in Nigeria with the goal of supporting the provision of incentives to optimize biological control usage in the region and assist scientists at the International Institute for Tropical Agriculture (IITA) in Nigeria in learning to apply the developed assays to assessment of aflatoxin-biocontrol end-user performance.

Approach:
The atoxigenic strains of A. flavus currently in development in Nigeria will be the focus of these studies. Techniques useful in detecting and quantifying use of the target atoxigenic strains on the maize crop will be developed to provide performance data based on crop sampling, isolation of deoxyribonucleic acid (DNA) and quantitative pyrosequencing. The work will include validation and refinement of assays, as well as, assistance in the training of collaborating Africa based scientists and the building of capacity in the target nation. Field and laboratory experience will be used to troubleshoot the assays and optimize them for the intended use in assessment of biological control activities.