Location: National Programs
Project Number: 0500-00053-003-60-G
Project Type: Grant
Start Date: Jul 1, 2013
End Date: Jun 30, 2017
(1) Identify and characterize other non-TRI cluster genes that are involved in protecting F. graminearum against trichothecene mycotoxins. (2) Test the feasibility of applying host-induced gene silencing (HIGS) to control FHB.
(1) a) identify and characterize genes that are specifically induced by DON (named DIG genes; 2) generate and identify DOS mutants that have increased sensitivity to DON; and 3) express the DIG and DOS genes in the budding yeast and determine their functions in conferring DON tolerance to yeast cells. (2) a) determine whether synthetic siRNA oligos can be effectively taken up by F. graminearum and result in the silencing of specific fungal genes; 2) use the VIGS (virus-induced gene silencing) approach to express silencing constructs of F. graminearum genes in wheat and examine their effects on fungal infection and DON production; and 3) determine whether treatments with combinations of siRNA oligos or co-expression of different BSMV VIGS constructs targeting at multiple genes have synergistic effects on reducing fungal growth, virulence, or DON production.