Location: National Programs
Project Number: 0500-00053-003-55-G
Project Type: Grant
Start Date: May 1, 2013
End Date: Apr 30, 2018
Project 1: The specific objectives of this proposal are to: 1) Continue to screen the Triticum timopheevii accessions obtained from NSGC for resistance to FHB in greenhouse; 2) Re-evaluate those accessions exhibiting a high level of resistance in the greenhouse; 3) Haplotype the resistant accessions identified and determine the novelty of the resistance sources. Project 2: Sreen advanced breeding lines/ elite germplasm selected by NDSU, SDSU, and UMN for FHB resistance (Type I and II) in the naturally-infected FHB nursery located at Jianyang, China. Project 3: Fine map the QTL, develop user-friendly DNA markers for their easy identification and transfer, and introgress the FHB resistance into adapted spring wheat varieties for better use in breeding programs.
(1) Use the point inoculation method to identify the type II resistance in these materials in the greenhouse and further confirm the potential resistance sources in greenhouse and field experiments. (2) Utilize an FHB nursery located at Jianyang, Fujian Province of China, is an excellent place for FHB evaluation because FHB epidemics occur every year under natural infection conditions. (3) a) Conduct saturation mapping of the two PI 277012-derived FHB resistance QTLs in the double haploid (DH) population (Grandin × PI 277012) using various molecular marker techniques, including SSR, STS, and SNP; b) Generate a large population of F2 progeny from the cross between PI 277012 (resistance to FHB) and the hard red spring wheat cultivar ‘Grandin’ (susceptible to FHB) and conduct fine mapping of the QTL in PI 277012 using the recombinant lines; c) Develop user-friendly DNA markers for marker assisted selection of the QTLs in breeding programs; and d) Introgress the FHB resistance from PI 277012 into adapted spring wheat varieties by crosses and backcrosses in combination with marker assisted selection and FHB evaluation.